棉花WD40基因的克隆及生物信息学分析(英文)

被引：4

作者：

谢永芳 ^{[1
,2
]}

蔡应繁 ^{[1
]}

江怀仲 ^{[1
]}

夏玉先 ^{[2
]}

孙全 ^{[1
]}

李生伟 ^{[1
]}

王伯初 ^{[2
]}

机构：

[1] 重庆邮电大学生物信息学院

[2] 重庆大学生物工程学院

来源：

Agricultural Science & Technology | 2008年 / 04期

关键词：

Cotton; WD40; Characterization;

D O I：

10.16175/j.cnki.1009-4229.2008.04.018

中图分类号：

S562 [棉];

学科分类号：

摘要：

A pair of PCR primers was designed based on the conserved sequences of WD40 family gene of different varieties.The normolization library was screened by PCR methods,the cDNA insert size of positive clones were analyzed by PCR method.A full-length cDNA of WD40(GenBank accession number:EU219610)in cotton was obtained from sequencing.This gene is 1 796 bp in length,containing an open reading frame encoding 274 amino acids and a stop codon from 35th to 860th position.The bioinformatics characterization indicates that the protein is encoded by WD40 domain.pI and molecular weight of the protein encoded were predicted to be 4.24 and 29 kDa,respectively.The yielded gene was accondingly named as GDRP1.RT-PCR analysis showed that the expression of GDRP1 varied during the gland formation process.These results will be helpful for our further study on the gland formation of cotton.

引用

页码：60 / 63

页数：4

共 3 条

[1] 强优势玉米杂交种雌穗均一化cDNA文库的构建与鉴定 [J].