吴茱萸碱对人胃癌SGC7901细胞增殖、侵袭和保罗样激酶-1基因表达的影响

被引：5

作者：

程兆明 ^{[1
]}

周永静 ^{[2
]}

张尤历 ^{[1
]}

祁卫东 ^{[2
]}

范钰 ^{[2
]}

机构：

[1] 江苏大学附属医院消化内科

[2] 不详

来源：

江苏大学学报(医学版) | 2011年 / 21卷 / 01期

关键词：

胃肿瘤; 吴茱萸碱; 侵袭; 保罗样激酶-1;

D O I：

10.13312/j.issn.1671-7783.2011.01.018

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

摘要：

目的:观察中草药提取物吴茱萸碱(evodiamine)对人胃癌细胞侵袭的影响及可能的机制。方法:采用不同浓度的吴茱萸碱处理人胃癌SGC7901细胞后,以软琼脂集落培养试验检测癌细胞锚着不依赖性增殖,以Boyden小室模型方法检测癌细胞侵袭能力;分别以荧光实时定量RT-PCR和免疫荧光方法检测癌细胞保罗样激酶-1(polo-likekinase 1,PLK1)基因表达水平。结果:胃癌SGC7901细胞经吴茱萸碱处理后,恶性增殖和穿膜细胞数均明显下降,且呈剂量依赖性(P<0.05)。吴茱萸碱处理组PLK1 mRNA和蛋白水平均明显下调,且呈时间和浓度依赖性(P<0.05)。结论:吴茱萸碱可明显抑制胃癌细胞侵袭力,其机制可能与下调PLK1基因表达有关。

引用

页码：69 / 72

页数：4

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