鱼腥草多酚对葡聚糖硫酸钠(DSS)诱导小鼠溃疡性结肠炎的改善作用

被引：21

作者：

董晶 ^{[1
]}

王帅珂 ^{[2
]}

吴苹 ^{[1
]}

王硕 ^{[1
]}

刘晋倩 ^{[1
]}

冀芦沙 ^{[1
]}

陈芳 ^{[1
]}

机构：

[1] 聊城大学药学院

[2] 聊城市人民医院转化医学研究联合实验室

来源：

现代食品科技 | 2021年 / 37卷 / 12期

关键词：

鱼腥草活性物; 溃疡性结肠炎; 葡聚糖硫酸钠; 炎症因子;

D O I：

10.13982/j.mfst.1673-9078.2021.12.0336

中图分类号：

TQ460.1 [基础理论];

学科分类号：

100705 [微生物与生化药学];

摘要：

该研究探讨了鱼腥草多酚对溃疡性结肠炎小鼠的改善效果。50只KM雄性小鼠随机分为空白组、模型组、鱼腥草多酚低、中、高剂量组。使用浓度为3.5%的葡聚糖硫酸钠(dextran sulphate sodium,DSS)溶液连续灌胃9 d制备小鼠肠炎模型。造模后第3 d灌胃给药,每天1次,连续10 d。观察小鼠体重变化、结肠组织形态学改变和疾病活动指数(disease active index,DAI);采用酶联法(ELISA)测定血清中炎症因子:肿瘤坏死因子(TNF-α),白细胞介素1β(IL-1β),白细胞介素(IL-6),γ干扰素(IFN-γ);试剂盒检测小鼠血清中肝肾生化指标:血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、血尿素氮(BUN)、血肌酐(Cr)。分析发现,与模型组相比,鱼腥草多酚干预组小鼠的便血减轻、结肠平均增加至8.35 cm(低剂量组)、9.53 cm(中剂量组)、10.87 cm(高剂量组)。DAI指数分别降低了29.83%、46.45%、54.82%。血清中TNF-α、IL-1β、IL-6、IFN-γ表达水平显著降低(p<0.01)。同时,鱼腥草多酚各剂量组降低了ALT、AST、Cr、BUN水平(p<0.05)。鱼腥草多酚可以有效改善DSS诱导KM雄性小鼠溃疡性结肠炎,降低血清促炎因子水平,其机制可能与抑制NF-κB相关。

引用

页码：7 / 13+229 +229

页数：8

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