通过卡那霉素选择体系再生可育小麦转基因植株

被引：14

作者：

徐子勤

D.Becker

H.Lrz

机构：

[1] 西北大学生物技术省重点实验室,InstitutfurAllgemeineBotanik,UniversitaHamburg,HamburgD-,Germany,InstitutfurAllgemeineBotanik,UniversitaHamburg,HamburgD-,Germany西安

来源：

自然科学进展 | 2001年 / 05期

关键词：

卡那霉素选择; 预培养; 高渗处理; 可育转基因小麦;

D O I：

暂无

中图分类号：

S512.1 [小麦];

学科分类号：

摘要：

使用卡那霉素作为选择剂建立起一套有效的小麦转化体系,预培养3d的未成熟胚在基因枪轰击前后进行高渗处理,轰击后于26℃、黑暗条件培养2周,然后用150mg/L硫酸卡那霉素选择培养2周,再转至含150mg/L硫酸卡那霉素的再生培养基进行光照培养。再生植株在温室中长出2～3片新叶后用2.5％硫酸卡那霉素水溶液喷洒;4周内,非转化体完全白化并枯萎,而转基因植株生长正常,用水稻Actinl启动子控制nptⅡ基因,卡那霉素选择体系的平均转化频率达到2.6％,高渗处理明显促进瞬间表达效果和稳定转化频率。目前已得到23个转基因小麦株系,大多数已经产生种子。Southern分析表明这些转基因株系均整合有不同拷贝的nptⅡ基因。

引用

页码：40 / 45

页数：6

共 16 条

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