解脲脲支原体多带抗原基因分型鉴定及其临床应用研究

被引：24

作者：

朱庆义

胡朝晖

刘峰林

刘懿微

郑建树

汪凡军

机构：

[1] 广州金域医学检验中心

[2] 佛山市顺德区均安医院

[3] 广州金域医学检验中心广东广州

[4] 广东广州

[5] 广东佛山

来源：

中华医院感染学杂志 | 2005年 / 08期

关键词：

解脲脲支原体; 多带抗原; 基因型; 聚合酶链反应;

D O I：

暂无

中图分类号：

R375 [支原体属];

学科分类号：

100103 ; 100705 ;

摘要：

目的应用PCR技术对解脲脲支原体(Uu)多带抗原进行分型鉴定,探讨Uu各种基因型与临床病原学之间的关系。方法根据解脲脲支原体多带抗原基因(MBA)与16S rRNA基因和尿素酶基因结构设计10对引物,采用PCR基因扩增技术,对384例非淋菌性尿道炎和其他泌尿生殖道感染患者的临床标本,进行解脲脲支原体生物变种和基因型分型鉴定,并与Uu培养法作比较。结果384例性病门诊患者临床标本中,检测Uu培养阳性218例,阳性率56.8%;PCR基因扩增检测Uu-DNA阳性208例,总阳性率为54.2%:其中生物变种1(biovar 1)143例,占37.2%,生物变种2(biovar 2)65例,占16.9%;基因分型结果:生物变种1血清变种1(serovar 1)50例,占13.0%,血清变种3/14(serovars 3/14)64例,占16.7%,血清变种6(serovar 6)29例,占7.6%;生物变种2亚型1(subtype 1)25例,占6.5%,亚型2(subtype 2)30例,占7.8%,亚型3(subtype 3)10例,占2.6%。结论Uu是性病的重要病原体,MBA多带抗原PCR基因分型鉴定具有简便、快速、敏感、特异之优点。

引用

页码：856 / 858+891 +891

页数：4

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