降香新黄酮latifolin通过Nrf2/HO-1通路抗H9c2细胞缺氧复氧损伤作用研究

被引：29

作者：

张妮 ^{[1
]}

陈兰英 ^{[1
]}

骆瑶 ^{[1
]}

罗颖颖 ^{[1
]}

崔亚茹 ^{[1
]}

刘荣华 ^{[2
]}

机构：

[1] 江西中医药大学国家工程研究中心

[2] 江西中医药大学药学院

来源：

中药材 | 2019年 / 42卷 / 07期

基金：

国家重点研发计划;

关键词：

降香; 新黄酮latifolin; 氧化应激; 缺氧复氧; Nrf2/HO-1通路;

D O I：

10.13863/j.issn1001-4454.2019.07.035

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:研究降香新黄酮latifolin对心肌H9c2细胞缺氧复氧损伤的保护作用及其可能机制。方法:建立H9c2细胞缺氧复氧损伤模型,实验分正常对照组、模型组及不同浓度latifolin(2.5、5、10、20μg/mL)组;采用MTT法检测细胞活力,微量酶标法测定细胞上清LDH活性;荧光染色法和流式细胞仪分析细胞中ROS含量;免疫荧光染色法和Western blot检测胞浆中Nrf2、HO-1及胞核中Nrf2蛋白表达。结果:与模型组比较,latifolin能显著升高H9c2细胞活力,降低细胞上清中LDH及细胞中ROS水平,升高胞浆中Nrf2、HO-1及胞核中Nrf2蛋白表达(P<0.05)。结论:latifolin对H9c2细胞缺氧复氧所致损伤具有保护作用,其机制可能与激活Nrf2/HO-1通路有关。

引用

页码：1629 / 1634

页数：6

共 9 条

[1]

降香新黄酮latifolin对大鼠急性心肌缺血影响及介导Nrf2信号通路机制研究.[J].李雪亮;陈兰英;官紫祎;骆瑶;崔亚茹;刘荣华;邵峰;王定清;.中国中药杂志.2017, 20

[2]

Ischemia/reperfusion injury and cardioprotective mechanisms: Role of mitochondria and reactive oxygen species [J].

Perrelli, Maria-Giulia ;

Pagliaro, Pasquale ;

Penna, Claudia .

WORLD JOURNAL OF CARDIOLOGY, 2011, 3 (06) :186-200

[3]

Olea europaea leaf extract up-regulates Nrf2/ARE/HO-1 signaling and attenuates cyclophosphamide-induced oxidative stress; inflammation and apoptosis in rat kidney.[J].Haifa A.S. ALHaithloul;Mohammed F. Alotaibi;May Bin-Jumah;Hassan Elgebaly;Ayman M. Mahmoud.Biomedicine & Pharmacotherapy.2019,

[4]

Betulinic acid alleviates myocardial hypoxia/reoxygenation injury via inducing Nrf2/HO-1 and inhibiting p38 and JNK pathways.[J].Dong Wang;Tingyan Chen;Fajun Liu.European Journal of Pharmacology.2018,

[5]

Maresin 1 Ameliorates Lung Ischemia/Reperfusion Injury by Suppressing Oxidative Stress via Activation of the Nrf-2-Mediated HO-1 Signaling Pathway.[J].Quanchao Sun;You Wu;Feng Zhao;Jianjun Wang;Massimo Collino.Oxidative Medicine and Cellular Longevity.2017,

[6]

Cardiac Oxidative Stress and Inflammatory Cytokines Response after Myocardial Infarction [J].

Neri, Margherita ;

Fineschi, Vittorio ;

Di Paolo, Marco ;

Pomara, Cristoforo ;

Riezzo, Irene ;

Turillazzi, Emanuela ;

Cerretani, Daniela .

CURRENT VASCULAR PHARMACOLOGY, 2015, 13 (01) :26-36

[7]

Cardioprotection by remote ischemic preconditioning of the rat heart is mediated by extracellular vesicles.[J].Zoltán Giricz;Zoltán V. Varga;Tamás Baranyai;Péter Sipos;Krisztina Pálóczi;Ágnes Kittel;Edit I. Buzás;Péter Ferdinandy.Journal of Molecular and Cellular Cardiology.2014,

[8]

Reperfusion injury in acute myocardial infarction. From bench to cath lab. Part I: Basic considerations.[J].Jean Pierre Monassier.Archives of Cardiovascular Diseases.2008, 7

[9]

中华人民共和国药典.[M].国家药典委员会.中国医药科技出版社.2015,

← 1 →