固相萃取-高效液相色谱法测定保健食品中维生素B12的研究

被引:26
作者
鲁杰
杨大进
王竹天
机构
[1] 中国疾控中心营养与食品安全所
[2] 中国疾控中心营养与食品安全所 北京
[3] 北京
关键词
维生素B12; 色谱法,高压液相; 营养保健品;
D O I
10.13590/j.cjfh.2004.04.008
中图分类号
TS207 [食品标准与检验];
学科分类号
083201 ;
摘要
为监管保健食品中维生素的含量 ,建立了保健食品中维生素B12 的化学分析法 ,采用固相萃取 (SPE)进行试样富集、净化 ,高效液相色谱 (HPLC)进行分离检测。方法的最小检出量为 0 3ng ,最低检出浓度 0 2mg kg ,线性范围在 0 334~ 16 7μg mL之间 ,平均回收率为 72 1%~ 131 7%。该方法具有简便、快速、可靠、容易推广等优点。
引用
收藏
页码:324 / 328
页数:5
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