针刺天枢穴对结肠炎肠上皮细胞NF-κB信号通路的影响

被引：9

作者：

李阳

郝艺照

傅熠俊

李赛美

机构：

[1] 广州中医药大学

来源：

中华中医药杂志 | 2017年 / 32卷 / 11期

关键词：

结肠炎; DSS; 天枢穴; 针刺疗法; 核因子-κB; 信号通路;

D O I：

暂无

中图分类号：

R245 [针灸学、针灸疗法];

学科分类号：

100517 [针灸学];

摘要：

目的:研究针刺"天枢穴"对DSS诱导结肠炎(IBD)的治疗作用及其对核因子-κB(NF-κB)信号通路的影响。方法:将30只C57BL/6小鼠随机分为空白组、模型组和天枢穴组,模型组和天枢穴组分别给予3%DSS水溶液造模,天枢穴组每日给予20min针刺干预,连续7d。记录每日DAI评分,取材后测量结肠长度,HE染色观察结肠病理变化,ELISA法检测结肠组织白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,Western blot法检测结肠组织中NF-κB、IκBα蛋白磷酸化水平,免疫荧光检测结肠组织中NF-κB表达水平。结果:天枢穴组每日DAI评分显著低于模型组(P<0.01);结肠长度针刺组显著长于模型组(P<0.05);结肠组织HE染色观察发现天枢穴组炎性损伤明显轻于模型组;结肠组织中IL-6、TNF-α含量天枢穴组显著低于模型组(P<0.01),NF-κB、IκBα磷酸化水平亦明显低于模型组(P<0.01)。结论:针刺天枢穴可以明显地改善DSS诱导IBD症状及结肠组织炎性反应损伤程度,可能是通过抑制NF-κB信号通路而起到了治疗作用,针刺天枢穴可以作为IBD一种有效的治疗手段。

引用

页码：5143 / 5147

页数：5

共 21 条

[1]

Gallic acid suppresses inflammation in dextran sodium sulfate-induced colitis in mice: Possible mechanisms [J].

Pandurangan, Ashok Kumar ;

Mohebali, Nooshin ;

Esa, Norhaizan Mohd ;

Looi, Chung Yeng ;

Ismail, Salmiah ;

Saadatdoust, Zeinab .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2015, 28 (02) :1034-1043

[2]

Oral administration of geraniol ameliorates acute experimental murine colitis by inhibiting pro-inflammatory cytokines and NF-κB signaling [J].

Medicherla, Kanakaraju ;

Sahu, Bidya Dhar ;

Kuncha, Madhusudana ;

Kumar, Jerald Mahesh ;

Sudhakar, Godi ;

Sistla, Ramakrishna .

FOOD & FUNCTION, 2015, 6 (09) :2984-2995

[3]

Dietary cocoa inhibits colitis associated cancer: a crucial involvement of the IL-6/STAT3 pathway [J].

Saadatdoust, Zeinab ;

Pandurangan, Ashok Kumar ;

Sadagopan, Suresh Kumar Ananda ;

Esa, Norhaizan Mohd. ;

Ismail, Amin ;

Mustafa, Mohd. Rais .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2015, 26 (12) :1547-1558

[4]

Wogonoside protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting NF-κB and NLRP3 inflammasome activation [J].

Sun, Yang ;

Zhao, Yue ;

Yao, Jing ;

Zhao, Li ;

Wu, Zhaoqiu ;

Wang, Yu ;

Pan, Di ;

Miao, Hanchi ;

Guo, Qinglong ;

Lu, Na .

BIOCHEMICAL PHARMACOLOGY, 2015, 94 (02) :142-154

[5]

Allicin Alleviates Dextran Sodium Sulfate- (DSS-) Induced Ulcerative Colitis in BALB/c Mice.[J].Ashok Kumar Pandurangan;Salmiah Ismail;Zeinab Saadatdoust;Norhaizan Mohd. Esa;Ryuichi Morishita.Oxidative Medicine and Cellular Longevity.2015,

[6]

The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation [J].