γ干扰素诱导气道黏液细胞凋亡及其机制的实验研究

被引：7

作者：

石昭泉

冯艳

侯元凯

刘涛

修清玉

机构：

[1] 第二军医大学第二附属医院呼吸科,江苏省宜兴市人民医院呼吸内科,解放军第十五医院呼吸科,黑龙江省克山县医院内科,第二军医大学第二附属医院呼吸科上海,上海

来源：

中华结核和呼吸杂志 | 2005年 / 03期

关键词：

黏液细胞; 化生; 哮喘; γ干扰素; 凋亡;

D O I：

暂无

中图分类号：

R562.25 [];

学科分类号：

摘要：

目的　研究γ干扰素(IFN-γ)对气道黏液细胞的作用及其机制。方法　 (1)体外培养正常人气道上皮细胞 5～7d后,以含 50ng/ml的IFN γ的培养基再培养 3d,采用异硫氰酸荧光素(FITC)免疫荧光和末端脱氧核苷酸转移酶介导的脱氧三磷酸尿嘧啶缺口末端标记 (TUNEL)方法,观察IFN-γ诱导体外培养人气道黏液细胞凋亡及其机制。(2) 20只野生型C57BL/6J小鼠经致敏后按数字表法随机分为 4组,每组 5只。分别经鼻气道内滴注IFN-γ50ng、IFN-γ100ng、白细胞介素 13(IL-13)5μg,并以生理盐水作对照,采用细胞形态学方法,分别计数各组致敏小鼠单位气道长度内黏液细胞数;采用TUNEL法检测气道黏液细胞的凋亡改变。结果　 ( 1 )免疫荧光细胞核染色联合TUNEL检测显示,经IFN γ作用后的人气道黏液细胞出现凋亡现象,细胞形态改变,胞核破裂;在细胞凋亡过程中,凋亡诱导因子Bax表达增加,并向线粒体转位。(2)经鼻向气管内滴注 100ngIFN γ后,小鼠气道单位长度(黏液细胞数 /毫米气道基底层,下同)黏液细胞数量为 (28±6)个 /mm,生理盐水组为(58±12)个 /mm,IL 13组为(59±6)个 /mm,生理盐水组和IL-13组与IFN γ100ng组比较差异有统计学意义(P<0.05);IFN γ50ng滴注组[ (48±11)个 /mm]与生理盐水组[ (58±12)个 /mm]和IL 13组[ (59±6)个

引用

页码：19 / 22

页数：4

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