表面等离子体子共振技术实时研究蛋白质在修饰表面的静电吸附行为

被引:7
作者
裴仁军
崔小强
杨秀荣
汪尔康
机构
[1] 中国科学院长春应用化学研究所电分析化学开放研究实验室国家电化学和光谱研究中心!长春
[2] 中国科学院长春应用化学研究所电分析化学开放研究实验室国家电化学和光谱研究中心!长
关键词
表面等离子体子共振; 静电吸附; 蛋白质;
D O I
暂无
中图分类号
O647.3 [吸附];
学科分类号
摘要
The elucidation of key influence factors for electrostatic adsorption is very important to control protein nonspecific adsorption on modified surfaces. In this study, real time surface plasmon resonance technique is used to characterize the electrostatic adsorption of two proteins (mouse IgG and protein A) on carboxymethyldextran modified surface. The results show that protein solution pH and ionic strength are key influence factors for efficient electrostatic adsorption. The influence of protein solution pH on the amount of electrostatic adsorption depends on the type of the charge and the charge density of both protein and modified matrix on the surface. The electrostatic adsorption process involves a competition between the positively charged protein and other positively charged species in the buffer solution. A decrease of ionic strength leads to an increasing electrostatic adsorption. The kinetic adsorption constants of protein A at different pH values were also calculated and compared.
引用
收藏
页码:1128 / 1130
页数:3
相关论文
共 2 条
[1]  
Johnsson B,Lofas S,Lindquist G. Anal. Biochem . 1991
[2]  
Jordan C E,Frutos A G,Thiel A J,et al. Anal Chem . 1997