细胞凋亡对中性粒细胞功能的影响

被引:5
作者
李英丽
陈泮藻
颜光涛
机构
[1] 解放军总医院基础所生化室!北京
关键词
中性粒细胞; 凋亡; 吞噬; 趋化; 活性氧生成;
D O I
暂无
中图分类号
Q25 [细胞生理学];
学科分类号
071009 ; 090102 ;
摘要
目的 :为了探讨中性粒细胞 (PMN)的自然凋亡和PMN凋亡对细胞吞噬、趋化和活性氧 (ROS)生成等功能的影响。方法 :采用光镜、电镜、电泳和流式细胞仪等研究手段探讨PMN的自然凋亡 ;通过葡萄球菌法、滤膜法和化学发光的方法研究凋亡PMN吞噬、趋化和ROS生成的改变。结果 :分离健康成人外周血PMN ,在体外进行 10 %血清培养 ,随着培养时间的延长 ,PMN凋亡率升高 ,同时PMN的吞噬、趋化以及ROS生成等功能都不同程度地受损。结论 :外周血PMN在体外培养发生自然凋亡 ,PMN的凋亡与细胞功能相关。
引用
收藏
页码:535 / 538
页数:4
相关论文
共 10 条
[1]  
Defects in intracellular oxidative metabolism of neutrophils undergoing apoptosis. Narayanan P K,Ragheb K,Lawler G et al. Journal of Leukocyte Biology . 1997
[2]  
Inhibition of activation-induced death in T cell hybridomas by thiol antioxidants: oxidative stress as a mediator of apoptosis. Sandstrom P A,Mannie M D,Buttke TM. Journal of Leukocyte Biology . 1994
[3]  
Neuron[P]. KUTKOVETSKYI VALENTYN YAKOVYCH;TURTI MARYNA VALENTYNIVNA.UA106289U,2016-04-25
[4]  
Apoptosis is associated with reduced expression of complement regulatory molecules, adhesion molecules and other receptors on polymorphonuclear leucocytes: functional relevance and role in inflammation. Jones J,Morgan B P. Immunology .
[5]  
Neutrophil apoptosis is associated with a reduction in CD16 (Fc gamma RIII) expression. Dransfield I,Buckle A M,Savill J S et al. J Immunol, 1994;153(3) .
[6]  
Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro. Homburg C H,de Haas M,von dem Borne AE et al. Blood . 1995
[7]  
Impairment of function in aging neutrophils is associated with apoptosis. Whyte M K B,Meagher L C,MacDermot T et al. J Immunol . 1993
[8]  
Inhibition of neutrophil apoptosis and prolongation of functional longevity by inflammatory mediators. Lee A,Whyte M K B,Haslett C et al. Journal of Leukocyte Biology . 1993
[9]  
Regulation of apoptosis in neutrophils-Fas track to death. Liles W C,Klebanoff S J. J Immunol, 1995;155 .
[10]  
Neutrophil superoxide release is required for spontaneous and FMLP-mediated but not for TNF alpha-mediated apoptosis. Kettritz R,Falk R J,Jennette J C et al. Journal of the American Society of Nephrology . 1997