Expression of Acyl-lipid 12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato

被引：11

作者：

Reza Maali Amiri ^{[1
]}

Natalia O Yureva ^{[2
]}

Khristina R Shimshilashvili ^{[3
]}

Irina V GoldenkovaPavlova ^{[3
]}

Vasiliy P Pchelkin ^{[2
]}

Elmira I Kuznitsova ^{[2
]}

Vladimir D Tsydendambaev ^{[2
]}

Tamara I Trunova ^{[2
]}

Dmitry A Los ^{[2
]}

Gholamreza Salehi Jouzani ^{[4
]}

Alexander M Nosov ^{[5
]}

机构：

[1] Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources of the University of Tehran

[2] KATimiryazev Institute of Plant Physiology, Russian Academy of Sciences (RAS)

[3] Vavilov Institute of General Genetics

[4] Agricultural Biotechnology Researches Institute of Iran (ABRII)

[5] Department of Plant Physiology, Moscow State University

来源：

JournalofIntegrativePlantBiology | 2010年 / 52卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q943.2 [植物基因工程]; S532 [马铃薯（土豆）];

学科分类号：

071007 [遗传学]; 090104 [作物信息科学与技术];

摘要：

We report the expression profile of acyl-lipid 12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coli) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.

引用

页码：289 / 297

页数：9

共 12 条

[1]

Hierarchy of sequence-dependent features associated with prokaryotic translation Lithwick G;Margalit H; Genome Res 2003,

[2]

Experimental Models for Creation of Transgenic PlantsResistant to Stressors[J] I. V. Goldenkova-Pavlova;N. Mirakhorli;A. R. Maali;E. Isaenko;N. A. Kartel’;N. O. Yur’eva;I. A. Abdeeva Cytology and Genetics 2007,

[3]

Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium[J] R. Maali-Amiri;I. V. Goldenkova-Pavlova;N. O. Yur’eva;V. P. Pchelkin;V. D. Tsydendambaev;A. G. Vereshchagin;A. N. Deryabin;T. I. Trunova;D. A. Los;A. M. Nosov Russian Journal of Plant Physiology 2007,

[4]

Antisense‐Mediated Depletion of Tomato Chloroplast Omega‐3 Fatty Acid Desaturase Enhances Thermal Tolerance[J] Xun‐Yan Liu;Jing‐Hua Yang;Bin Li;Xiu‐Mei Yang;Qing‐Wei Meng Journal of Integrative Plant Biology 2006,

[5]

Modification of the sunflower defensin SD2 gene sequence and its expression in bacterial and yeast cells[J] D. V. Sotchenkov;I. V. Goldenkova;N. Miraholi;L. V. Volkova Russian journal of genetics 2005,

[6]

Thermostable lichenase as a translational reporter[J] R. A. Komakhin;I. A. Abdeeva;G. R. Salehi Dzhuzani;I. V. Goldenkova;A. A. Zhuchenko Russian Journal of Genetics 2005,

[7]

Comparative study of the expression of the native; modified; and hybrid cry3a genes of Bacillus thuringiensis in prokaryotic and eukaryotic cells[J] G. R. Salehi Jozani;R. A. Komakhin;E. S. Piruzian Russian journal of genetics 2005,

[8]

The Effect of Tobacco Plant Transformation with a Gene for Acyl-Lipid Δ9-Desaturase from <Emphasis Type="Italic">Synechococcus vulcanus</Emphasis> on Plant Chilling Tolerance[J] V. N. Popov;I. V. Orlova;N. V. Kipaikina;T. S. Serebriiskaya;N. V. Merkulova;A. M. Nosov;T. I. Trunova;V. D. Tsydendambaev;D. A. Los Russian Journal of Plant Physiology 2005,

[9]

Bifunctional Reporter Genes: Construction and Expression in Prokaryotic and Eukaryotic Cells[J] I. V. Goldenkova;K. A. Musiychuk;E. S. Piruzian Molecular Biology 2003,

[10]

Construction of Synthetic Genes for Analogs of Spider Silk Spidroin 1 and Their Expression in Tobacco Plants[J] E. S. Piruzian;V. G. Bogush;K. V. Sidoruk;I. V. Goldenkova;K. A. Mysiychuk;V. G. Debabov Molecular Biology 2003,

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