糖尿病与非糖尿病性脑梗死患者MTHFR基因多态性C677T与同型半胱氨酸水平相关性研究

被引：25

作者：

马亮 ^{[1
]}

刘倩 ^{[1
]}

丛笑 ^{[1
]}

姜永玮 ^{[1
]}

朋毛措 ^{[2
]}

韩呈武 ^{[1
]}

湛玉良 ^{[1
]}

曹永彤 ^{[1
]}

机构：

[1] 北京, 中日友好医院检验科

[2] 青海省第四人民医院检验科

来源：

中华检验医学杂志 | 2016年 / 39卷 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

脑梗死; 亚甲基四氢叶酸还原酶; 高半胱氨酸; 糖尿病; 多态现象, 遗传;

D O I：

暂无

中图分类号：

R587.2 [糖尿病性昏迷及其他并发症]; R743.33 [脑栓塞];

学科分类号：

1002 ; 100201 ;

摘要：

目的探讨脑梗死患者亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性(rs1801133)特点及其与同型半胱氨酸(Hcy)水平相关性, 并进一步分析糖尿病性(DM)与非糖尿病性(NDM)脑梗死患者rs1801133多态性特点和Hcy水平。方法病例对照研究。选取2014年1月至2015年1月中日友好医院脑梗死住院患者556例及体检中心正常对照者275名, 采用焦磷酸测序技术检测MTHFR基因C677T多态性, 采用循环酶法检测血清Hcy水平。对不同组别MTHFR基因型分布采用卡方检验进行统计学分析;脑梗死患者不同基因型之间Hcy水平比较采用ANVOA检验, 进一步两两比较采用LSD-t检验。结果 556例脑梗死患者中TT型202例(36.33%), CT型257例(46.22%), CC型97例(17.45%), 等位基因T频率为59.44%明显高于正常对照组46.91%(χ2=23.385, P<0.001)。TT型脑梗死患者Hcy水平(21.31±17.31)μmol/L, 明显高于CT基因型患者(14.88±7.71) μmol/L(P<0.001)和CC基因型患者(14.48±7.78)μmol/L(P<0.001)。糖尿病性脑梗死组MTHFR基因TT型频率(36.77%)与非糖尿病性脑梗死组频率(36.44%)相比, 差异无统计学意义(χ2=0.031, P>0.05);该基因型糖尿病性脑梗死组Hcy浓度为(18.16±12.90)μmol/L, 显著低于非糖尿病性脑梗死组的(23.47±19.53 )μmol/L(F=4.652, P<0.05)。结论 MTHFR基因TT型与血清高Hcy水平有关, 可能是脑梗死发生的危险因素;TT基因型糖尿病性脑梗死患者相对于该基因型非糖尿病性脑梗死患者具有更低的Hcy水平。(中华检验医学杂志, 2016, 39:205-209)

引用

页码：205 / 209

页数：5

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