地塞米松对实验性哮喘小鼠TH1/TH2细胞因子的影响及其机制的探讨

被引：6

作者：

潘世秀 ^{[1
]}

曾天舒 ^{[2
]}

崔天盆 ^{[1
]}

胡丽华 ^{[1
]}

吴健民 ^{[1
]}

机构：

[1] 华中科技大学同济医学院附属协和医院检验科

[2] 华中科技大学同济医学院附属协和医院内分泌科

来源：

中国病理生理杂志 | 2007年 / 06期

关键词：

哮喘; 白细胞介素-4; 干扰素Ⅱ型; 地塞米松; GATA-3; T-bet;

D O I：

暂无

中图分类号：

R562.25 [];

学科分类号：

摘要：

目的:初步探讨地塞米松对小鼠哮喘模型哮喘进展过程中TH细胞因子的影响及其机制。方法:18只BALB/c小鼠随机分为正常对照组、哮喘组和地塞米松处理组,每组各6只。运用流式细胞仪检测小鼠脾细胞胞内细胞因子白细胞介素-4和干扰素-γ的表达,用Western blotting方法检测各组小鼠肺组织转录因子T-bet和GATA-3的表达,用组织学观察肺组织炎症程度。结果:哮喘鼠T细胞内IL-4/IFN-γ比值明显高于正常组(P(0.01);地塞米松组细胞内IL-4/IFN-γ比值明显低于哮喘组(P(0.01)。哮喘组肺组织T-bet的表达明显低于正常对照组(P(0.01),GATA-3明显高于正常对照组(P(0.01);地塞米松组的小鼠肺组织T-bet和GATA-3的表达均明显低于哮喘组(P(0.01),GATA-3降低更为明显。结论:调控TH1和TH2细胞转化过程中重要转录因子T-bet和GATA-3的表达,可以改变IL-4/IFN-γ比值,纠正哮喘的TH2漂移,从而改善相应的炎性症状,这可能是地塞米松抑制哮喘的重要机制之一。

引用

页码：1141 / 1144

页数：4

共 8 条

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