壳聚糖涂层亲和层析在超氧化物歧化酶纯化中的应用

被引：5

作者：

谭天伟

桑晋

机构：

[1] 北京化工大学化学工程学院生物化工系!北京

来源：

高校化学工程学报 | 1998年 / 02期

关键词：

壳聚糖; 固定化金属离子亲和层析; 超氧化物歧化酶;

D O I：

暂无

中图分类号：

O658 [元素及化合物的分离方法];

学科分类号：

070302 ; 081704 ;

摘要：

A new adsorbent for immobilized metal ion affinity chromatography (IMAC) was stud-ied. The preparation of an adsorbent with coated chitosan on silica beads Was made. After being cross-linked by dechloride diglycidyl ether, the chitosan coated adsorbent had good chemical stability andhigh mechanic property. When pH> 3. 8, the Cu2+ was adsorbed easily on the chitosan coated adsor-bent with adorbed capacity of 18 mg Cu2+/g chitosan or 0. 91 mg Cu2+ /g beads. The new adsorbentcouId be used for purification of superoxide dismutase. The recovery of SOD was 89% with a purifi-cation factor of 20.

引用

页码：189 / 192

页数：4