快速老化小鼠胸腺细胞分化与Notch信号相关基因表达关系的研究

被引：3

作者：

毕明刚

周文霞

齐春会

张永祥

机构：

[1] 军事医学科学院毒物药物研究所

来源：

中国免疫学杂志 | 2005年 / 12期

关键词：

胸腺细胞; 信号转导; 细胞分化; Notch; Presenilin; HES;

D O I：

暂无

中图分类号：

R392.1 [免疫生物学];

学科分类号：

摘要：

目的研究快速老化小鼠(Senescence-accelerated mice,SAM)增龄过程中胸腺T细胞亚群的变化与Notch1、Prese-nilin1、2(PS1、2)、HES1等Notch信号转导通路相关基因mRNA表达变化及这两种变化的关系。方法选择出生后1～8周龄SAM,采用流式细胞技术检测胸腺细胞亚群变化、用荧光实时定量PCR的方法检测Notch1、PS1、PS2、HES1基因mRNA表达的动态变化。结果SAM出生1～2周,胸腺中CD4+CD8+细胞所占比例较高,4周龄后逐渐降低,各周龄SAMP8与SAMR1之间无显著性差异;在CD4+CD8-细胞比例上,SAM呈增龄性增加,1～6周龄的SAMP8与SAMR1之间无显著性差异,8周龄的SAMP8低于SAMR1;在CD4-CD8+细胞比例上,SAM呈增龄性增加,4周龄后的SAMP8均明显高于SAMR1;Notch1、PS2、HES1基因mR-NA表达量呈增龄性增加,各周龄SAMP8的Notch1基因表达水平高于SAMR1,4周龄后SAMP8的PS2、HES1基因表达水平高于SAMR1;PS1基因mRNA表达量呈增龄性降低,4周龄后的SAMP8低于SAMR1。结论Notch1、PS2、HES1的表达与胸腺CD4-CD8+细胞的分化呈正相关,PS1表达与胸腺CD4-CD8+细胞的分化呈负相关。

引用

页码：905 / 909

页数：5

共 20 条

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