吴茱萸碱刺激的负载脊髓匀浆蛋白DCs促进小鼠脊髓损伤的修复

被引：4

作者：

王科 ^{[1
]}

赵建华 ^{[1
]}

梁华平 ^{[2
]}

机构：

[1] 第三军医大学大坪医院野战外科研究所骨科

[2] 第三军医大学大坪医院第一研究室,创伤、烧伤与复合伤国家重点实验室

来源：

第三军医大学学报 | 2010年 / 32卷 / 03期

关键词：

脊髓损伤; 树突状细胞; 吴茱萸碱; 脊髓匀浆蛋白;

D O I：

10.16016/j.1000-5404.2010.03.001

中图分类号：

R285.5 [中药实验药理];

学科分类号：

摘要：

目的探讨吴茱萸碱(evodiamine,EVO)刺激的负载脊髓匀浆蛋白(homogenate protein of spinal cord,hp)树突状细胞(hpDCevo)对小鼠脊髓损伤(spinal cord injury,SCI)的促修复作用及其机制。方法SCI后24 h给予治疗,随机分6组:分别腹腔注射PBS、EVO、未成熟DC(DCs)、经EVO刺激的DCs(DCevo)、负载hp的DC(hpDC)、hpDCevo。利用BMS功能评分及组织病理学方法,观察hpDCevo对小鼠脊髓损伤神经功能恢复和局部瘢痕等病理学改变的影响。ELISA法测定SCI损伤局部组织和T细胞培养上清中的脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、神经营养因子-3(Neurotrophin-3,NT-3)、白介素-12(interleukin-12,IL-12)、粒-巨噬细胞集落刺激因子(granulocyte-macrophage colonystimulating factor,GM-CSF)的含量。结果注射后第84天,hpDCevo组BMS评分明显高于hpDC组和其余各组(P<0.05)。组织病理学结果显示,hpDCevo组较其他组空洞和瘢痕缩小。损伤局部组织中的BDNF、NT-3、GM-CSF、IL-12含量为hpDCevo组高于hpDC组和其余各组(P<0.05);T细胞培养上清中的BDNF、GM-CSF为hpDCevo组高于hpDC组和其余各组(P<0.05)。结论hpDCevo对小鼠SCI修复有促进作用,其机制可能是通过上调损伤局部的BDNF、NT-3、GM-CSF、IL-12来实现的。

引用

页码：269 / 273

页数：5

共 11 条

[1] 基因芯片检测吴茱萸碱对小鼠DC细胞功能调控相关基因表达的影响
祝绚
梁华平
鲍依稀
[J]. 中国免疫学杂志, 2008, (07) : 597 - 600
[2] Boosting T-cell immunity as a therapeutic approach for neurodegenerative conditions: The role of innate immunity[J] . M. Schwartz,A. London,R. Shechter.Neuroscience . 2009 (3)
[3] Functional recovery after spinal cord injury in mice through activation of microglia and dendritic cells after IL-12 administration
Yaguchi, Masae
Ohta, Shigeki
Toyama, Yoshiaki
Kawakami, Yutaka
Toda, Masahiro
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2008, 86 (09) : 1972 - 1980
[4] Protective autoimmunity as a T-cell response to central nervous system trauma: prospects for therapeutic vaccines[J] . Michal Schwartz.Progress in Neurobiology . 2001 (5)
[5] Autoimmune T cells as potential neuroprotective therapy for spinal cord injury
Hauben, E
Nevo, U
Yoles, E
Moalem, G
Arganov, E
Mor, F
Akselrod, S
Neeman, M
Cohen, IR
Schwartz, M
[J]. LANCET, 2000, 355 (9200) : 286 - 287
[6] Production of Neurotrophins by Activated T Cells: Implications for Neuroprotective Autoimmunity[J] . Gila Moalem,Amos Gdalyahu,Yael Shani,Uwe Otten,Philip Lazarovici,Irun R Cohen,Michal Schwartz.Journal of Autoimmunity . 2000 (3)
[7] Autoimmune T cells retard the loss of function in injured rat optic nerves[J] . Gila Moalem,Eti Yoles,Raya Leibowitz-Amit,Shir Muller-Gilor,Felix Mor,Irun R Cohen,Michal Schwartz.Journal of Neuroimmunology . 2000 (1)
[8] An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow
Lutz, MB
Kukutsch, N
Ogilvie, ALJ
Rössner, S
Koch, F
Romani, N
Schuler, G
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 223 (01) : 77 - 92
[9] Truncated trkB receptors on nonneuronal cells inhibit BDNF-induced neurite outgrowth in vitro
Fryer, RH
Kaplan, DR
Kromer, LF
[J]. EXPERIMENTAL NEUROLOGY, 1997, 148 (02) : 616 - 627
[10] ASSESSMENT OF AXONAL DYSFUNCTION IN AN IN-VITRO MODEL OF ACUTE COMPRESSIVE INJURY TO ADULT-RAT SPINAL-CORD AXONS
FEHLINGS, MG
NASHMI, R
[J]. BRAIN RESEARCH, 1995, 677 (02) : 291 - 299

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