产志贺毒素大肠杆菌多重PCR与Vero细胞毒实验对比研究

被引:3
作者
马宏
王建丽
王殿法
徐强
傅炳南
涂光理
机构
[1] 河南省卫生防疫站
[2] 商丘市卫生防疫站
[3] 睢县卫生防疫站
[4] 河南省卫生防疫站 郑州
[5] 郑州
[6] 河南
关键词
多重PCR; Vero细胞毒实验; 志贺毒素;
D O I
暂无
中图分类号
R446.5 [微生物学检验];
学科分类号
100208 ;
摘要
[目的 ]通过对比研究建立一种具有高特异性、敏感性的检测产志贺毒素大肠杆菌 (STEC)的实验方法。 [方法 ]应用多重 PCR和 Vero细胞毒实验方法 ,对比检测从疫区分离的 142株大肠杆菌菌株。 [结果 ]6 0株多重 PCR stx2或 stx1 阳性大肠杆菌 Vero细胞毒试验均阳性。 82株不含有 stx2 或 stx1 基因大肠杆菌 Vero细胞毒试验均阴性。两者试验结果完全吻合。 [结论 ]多重 PCR方法检测 STEC stx2 和 stx1 具有高度的特异性和敏感性 ,可作为鉴定大肠杆菌是否为 STEC的首选方法
引用
收藏
页码:7 / 10
页数:4
相关论文
共 12 条
[1]  
Karmali MA,Petric M,Lim C,et al.The association between Idiopathic Hemolytic Uremic Syndrome and infection by verotoxinproducing Escherichia coli. The Journal of Infectious Diseases . 1985
[2]  
Mead PS,Griffin PM.Escherichia coli O157: H7. The Lancet . 1998
[3]  
Bingnan Fu,Unicomb LE,Guangli Tu,et al.Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specifities, and sertype 2 VP7 gens. Journal of Clinical Microbiology . 1991
[4]  
Karch H,Janetzki -Mittmann C,Aleksic S,et al.Isolation of enterohemorrhagic Escherichia coli O157∶H7 strains from patie-nts with Hemolytic-Uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture. Journal of Clinical Microbiology . 1996
[5]  
Paton AW,Paton JC.Direct detection of shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR. Journal of Clinical Microbiology . 1999
[6]  
Karmali MA,Petric M,Lim C,et al.Sensitive method for detecting low number of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin. Journal of Clinical Microbiology . 1985
[7]  
Riley LW,Remis RS,Helgerson SD.Heamorrhagic colitis associated with a rare Echerichia coli serotype. The New England Journal of Medicine . 1983
[8]  
Karmali MA,Steele BT,Petric M et al.Sporadic cases of hemolytic uremic syndrome associated with faecal cytotoxin-producing Echerichia coli in stools. The Lancet . 1983
[9]  
Tschape H,Prager R,Streckel W,et al.Verotoxinogenic Citrobacter Freundii associated with severe gastroenteritis and cases of haemolytic uraemic syndrome in a nursery school : gree butter as the infection source. Epidemiology and Infection . 1995
[10]  
Beutin L,Zimmermann S,Gleier K.Rapid detection and isolation of Shige-like toxin ( Verocytotoxin )-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay. Journal of Clinical Microbiology . 1996