缺血后处理对脑缺血再灌注损伤后ERK1/2和Akt磷酸化及神经细胞凋亡的影响

被引：17

作者：

邢变枝 ^{[1
]}

陈晖 ^{[2
]}

张苏明 ^{[3
]}

机构：

[1] 武汉大学人民医院放射科

[2] 武汉大学人民医院泌尿外科

[3] 华中科技大学同济医学院附属同济医院神经内科

来源：

神经损伤与功能重建 | 2012年 / 7卷 / 03期

关键词：

缺血后处理; ERK1/2; Akt; 脑缺血再灌注损伤;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

摘要：

目的:观察缺血后处理对大鼠局灶性脑缺血再灌注损伤后ERK1/2和Akt及神经细胞凋亡的影响。方法:成年健康SD大鼠72只,随机分为假手术(sham)组、缺血再灌注(I/R)组、缺血后处理(Postcond)组各24只,应用线栓法建立大脑中动脉闭塞(MCAO)再灌注模型。分别于再灌注10min、30min、6h、24h后留取大脑皮质。Western blot检测再灌注10min、30min、6h后ERK1/2和Akt活性变化;原位末端标记(TUNEL)检测再灌注后24h神经细胞凋亡。结果:Postcond组再灌注10min、30min、6h后ERK1/2和Akt活性高于I/R组(P<0.05);脑缺血再灌注24h后,Postcond组与I/R组比较,TUNEL阳性细胞减少(P<0.05)。结论:缺血后处理可提高大鼠脑缺血再灌注后皮质内ERK1/2和Akt活性,减少神经细胞凋亡。

引用

页码：175 / 179

页数：5

共 11 条

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