阿霉素诱导的心力衰竭小鼠心脏去甲肾上腺素转运蛋白的表达变化

被引：13

作者：

靳文英

乔正国

郑春华

李素芳

陈红

机构：

[1] 北京大学人民医院心脏中心

来源：

中国循环杂志 | 2013年 / 28卷 / 06期

关键词：

去甲肾上腺素转运蛋白; 阿霉素; 充血性心力衰竭; 交感神经;

D O I：

暂无

中图分类号：

R541.6 [血液循环衰竭];

学科分类号：

100201 [内科学];

摘要：

目的:观察阿霉素诱导的充血性心力衰竭(心衰)小鼠心脏去甲肾上腺素转运蛋白(NET)的表达变化。方法:建立阿霉素诱导的充血性心衰小鼠动物模型,分离小鼠心脏和颈交感神经节,实时荧光定量多聚酶链反应(PCR)的方法检测NET和肾上腺素能受体β1-AR的mRNA表达,用Western blot方法检测NET和酪氨酸羟化酶(TH)的蛋白表达。免疫组化方法染色心脏交感神经纤维。结果:①心衰小鼠心脏交感神经节中NET和β1-AR的mRNA表达无明显变化。②心衰小鼠心肌组织中NET的蛋白表达显著降低,而TH的蛋白表达上调。③心衰早期小鼠心肌交感神经纤维密度无明显变化。结论:充血性心衰小鼠心脏NET的蛋白表达存在转录后的下调,心衰早期NET的表达下调可导致NE再摄取障碍,交感神经活性失衡从而加剧心功能的恶化。

引用

页码：458 / 462

页数：5

共 11 条

[1]

β受体阻滞剂在心力衰竭治疗中的应用 [J].

张健 .

中国循环杂志, 2009, 24 (05) :391-393

[2]

Cardiac sympathetic nerve terminal function in congestive heart failure[J] Chang-seng LIANG School of Medicine and Dentistry;University of Rochester;Rochester;New York 14642;USA Acta Pharmacologica Sinica 2007, 07

[3]

Norepinephrine transporter (NET) is expressed in cardiac sympathetic ganglia of adult rat[J] LI HE; SUN KAI MA ; XIAO PING HU ; GOU YUAN ZHANG ; JIAN FEI Institute of Biochemistry and Cell Biology; Shanghai Institutes for Biological Sciences; Chinese Academy of Sciences; Shanghai 200031; China Cardiovascular Division of Changzheng Hospital; Shang Cell Research 2001, 04

[4]

Carvedilol Improves Uptake-1 in Patients With Systolic Congestive Heart Failure[J] Maiko Machida;Shigeru Takechi;Tetsuya Fujimoto;Shigeo Kakinoki;Akikazu Nomura Journal of Cardiovascular Pharmacology 2012,

[5]

Adrenergic Excess; hNET1 Down-Regulation; and Compromised m IBG Uptake in Heart Failure[J] Nezam Haider;Ragavendra R. Baliga;Y. Chandrashekhar;Jagat Narula JACC: Cardiovascular Imaging 2010,

[6]

Altered Sympathetic Nervous Reactivity and Norepinephrine Transporter Expression in Patients With Postural Tachycardia Syndrome[J] Elisabeth Lambert;Nina Eikelis;Murray Esler;Tye Dawood;Markus Schlaich;Richard Bayles;Flora Socratous;Alex Agrotis;Garry Jennings;Gavin Lambert;Gautam Vaddadi Circulation: Arrhythmia and Electrophysiology 2008,

[7]

Injection of Nerve Growth Factor Into Stellate Ganglia Improves Norepinephrine Reuptake Into Failing Hearts[J] Michael M. Kreusser;Markus Haass;Sebastian J. Buss;Stefan E. Hardt;Stefan H. Gerber;Ralf Kinscherf;Hugo A. Katus;Johannes Backs Hypertension 2006,

[8]

Cardiac Overexpression of the Norepinephrine Transporter Uptake-1 Results in Marked Improvement of Heart Failure[J] Circulation Research 2005,

[9]

The Neuronal Norepinephrine Transporter in Experimental Heart Failure: Evidence for a Posttranscriptional Downregulation[J] Johannes Backs;Armin Haunstetter;Stefan H. Gerber;Jürgen Metz;Mathias M. Borst;Ruth H. Strasser;Wolfgang Kübler;Markus Haass Journal of Molecular and Cellular Cardiology 2001,

[10]

The role of neuronal and extraneuronal plasma membrane transporters in the inactivation of peripheral catecholamines[J] Graeme Eisenhofer Pharmacology and Therapeutics 2001,

← 1 2 →