菊苣酸对脓毒症大鼠心肌损伤时氧化应激的影响及其与Nrf2信号通路的关系

被引：9

作者：

李依玲 ^{[1
]}

姚鹏 ^{[2
]}

祁皓 ^{[2
]}

卢忆萍 ^{[1
]}

毛毅 ^{[1
]}

孟杰 ^{[1
]}

肖航

机构：

[1] 武汉科技大学附属孝感医院麻醉科

[2] 武汉科技大学附属孝感医院重症医学科

来源：

中华麻醉学杂志 | 2021年 / 41卷 / 04期

关键词：

咖啡酸类; 脓毒症; 心脏损伤; 氧化性应激; NF-E2相关因子2;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的评价菊苣酸对脓毒症大鼠心肌损伤时氧化应激的影响及其与核因子E2相关因子2(Nrf2)信号通路的关系。方法健康雄性SD大鼠40只, 8～12周龄, 体重220～250 g, 采用随机数字表法分为5组(n=8):对照组(C组)、LPS组、LPS+菊苣酸组(LPS+CA组)、LPS+Nrf2抑制剂ML385组(LPS+ML组)和LPS+菊苣酸+ML385组(LPS+CA+ML组)。腹腔注射LPS 15 mg/kg制备大鼠脓毒症心肌损伤模型。腹腔注射LPS即刻, LPS+CA组和LPS+ML组分别腹腔注射菊苣酸10 mg/kg或ML385 15 mg/kg(均用DMSO溶解), LPS+CA+ML组腹腔注射ML385 15 mg/kg和菊苣酸10 mg/kg, C组腹腔注射等容量DMSO。造模后48 h时, 取主动脉血样, 采用ELISA法测定血清IL-6浓度、LDH和CK-MB活性。然后处死大鼠, 取心肌组织, HE染色后光镜下观察病理学结果, 采用化学比色法测定谷胱甘肽过氧化物酶(GSH-Px)和SOD活性、ROS和铁含量, 计算NAD+/NADH比值, 采用Western blot法测定Nrf2、醌氧化还原酶1(NOQ1)、谷胱甘肽过氧化物酶4(GPX4)和尼克酰胺腺嘌呤二核苷酸磷酸氧化酶1(NOX1)的表达水平。结果与C组相比, 其余4组血清LDH、CK-MB活性和IL-6浓度升高, 心肌组织ROS和铁含量及NAD+/NADH比值升高, GSH-Px和SOD活性降低, Nrf2、NQO1和GPX4表达下调, NOX1表达上调(P<0.05);与LPS组相比, LPS+CA组血清LDH、CK-MB含量和IL-6浓度降低, 心肌组织ROS和铁含量及NAD+/NADH比值降低, GSH-Px和SOD活性升高, Nrf2、NQO1和GPX4表达上调, NOX1表达下调(P<0.05), 心肌细胞病理学损伤明显减轻, LPS+ML组血清LDH、CK-MB含量和IL-6浓度升高, 心肌组织NAD+/NADH比值升高, GSH-Px和SOD活性降低, NQO1和GPX4表达下调, NOX1表达上调(P<0.05), 心肌细胞病理学损伤进一步加重;与LPS+CA组相比, LPS+CA+ML组血清LDH、CK-MB活性和IL-6浓度升高, 心肌组织ROS和铁含量及NAD+/NADH比值升高, GSH-Px和SOD活性降低, Nrf2、GPX4和NQO1表达下调, NOX1表达上调(P<0.05), 心肌细胞病理学损伤明显加重。结论菊苣酸减轻脓毒症大鼠心肌损伤的机制可能与激活Nrf2信号通路, 抑制氧化应激有关。

引用

共 19 条

[1]

菊苣酸对脂多糖诱导的H9C2细胞损伤的保护作用及机制 [J].

李依玲 ;

邓伟 ;

殷炎燕 ;

余树春 .

中国急救医学, 2018, 38 (12) :1095-1098

[2]

Melatonin improves hypoxic-ischemic brain damage through the Akt/Nrf2/Gpx4 signaling pathway[J] Zhixian Gou;Xiaojuan Su;Xing Hu;Yue Zhou;Lin Huang;Yang Fan;Jing Li;Liqun Lu Brain Research Bulletin 2020,

[3]

Aerobic exercise modulates cardiac NAD(P)H oxidase and the NRF2/KEAP1 pathway in a mouse model of chronic fructose consumption.[J] Alves Renata;Suehiro Camila Liyoko;Oliveira Flavia Garcia de;Frantz Eliete Dalla Corte;Medeiros Renata Frauches de;Vieira Rodolfo de Paula;Martins Milton de Arruda;Lin Chin Jia;Nobrega Antonio Claudio Lucas da;Toledo-Arruda Alessandra Choqueta de Journal of applied physiology (Bethesda; Md. : 1985) 2020,

[4]

Nrf2 Suppression Delays Diabetic Wound Healing Through Sustained Oxidative Stress and Inflammation[J] Frontiers in Pharmacology 2019,

[5]

Chicoric acid prevents methotrexate-induced kidney injury by suppressing NF-κB/NLRP3 inflammasome activation and up-regulating Nrf2/ARE/HO-1 signaling.[J] Abd El-Twab Sanaa M;Hussein Omnia E;Hozayen Walaa G;Bin-Jumah May;Mahmoud Ayman M Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2019,

[6]

Sepsis: Inflammation Is a Necessary Evil[J] Christina Nedeva;Joseph Menassa;Hamsa Puthalakath Frontiers in Cell and Developmental Biology 2019,

[7]

Schisandrin B Attenuates Inflammation in LPS-Induced Sepsis Through miR-17-5p Downregulating TLR4.[J] Ji Zhi-Rong;Xue Wei-Liang;Zhang Ling Inflammation 2019,

[8]

Carcinogenesis and Reactive Oxygen Species Signaling: Interaction of the NADPH Oxidase NOX1-5 and Superoxide Dismutase 1-3 Signal Transduction Pathways.[J] Parascandolo Alessia;Laukkanen Mikko O Antioxidants & redox signaling 2019,

[9]

Involvement of Nrf2 in myocardial ischemia and reperfusion injury[J] Yiming Shen;Xiaojuan Liu;Jiahai Shi;Xiang Wu International Journal of Biological Macromolecules 2018,

[10]

Schizandrin B protects LPS-induced sepsis via TLR4/NF-κB/MyD88 signaling pathway.[J] Xu Jianjun;Lu Caijiao;Liu Zhengjun;Zhang Peng;Guo Hailei;Wang Tingting American journal of translational research 2018,

← 1 2 →