Induction of Angiogenesis and Neurogenesis by Serum from Rats Treated with Shunaoxin Dropping Pills

Objective Shunaoxin Dropping Pills(SDPs),a Chinese patent medicine,has been used widely in China for the treatment of headache,amnesia,and insomnia.The aim of the present study is to observe the effect of SDPs on inducing angiogenesis and neurogenesis in vitro.Methods The present testing system using the serum obtained from animals ig treated with SDPs and a co-culture system in vitro was used to investigate if SDPs promotes brain microvascular endothelial cells(BMECs)tube formation and neural differentiation of neural stem/progenitor cells(NSPCs),which plays important roles in angiogenesis and neurogenesis.Results The SDPs serum sampled from rats ig treated with SDPs for 3 d dose-dependently promoted the tube like structure formation of cultured BMECs,and enhanced the fraction of MAP-2 positive cells of NSPCs,which co-cultured with the BMECs and astrocyte.In addition,there was no significant change in the percentage of glial fibrillary acidic protein positive cells.Conclusion Our results show that SDPs serum can induce neural differentiation and BMECs tube formation in vitro.