汉族人群血管紧张素原基因的新单核苷酸多态性位点

被引:6
作者
刘天承
侯淑琴
张琳
杜志荣
余跃
邱长春
机构
[1] 中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室
[2] 北京市房山区第一医院
[3] 中国医学科学院
基金
北京市自然科学基金;
关键词
单链构象多态性; 单核苷酸多态性; 非变性聚丙烯酰胺凝胶电泳;
D O I
暂无
中图分类号
R346 [];
学科分类号
1001 ;
摘要
应用改进的PCR SSCP技术、多种聚丙烯酰胺凝胶电泳和PCR产物直接测序等方法 ,分析人的血管紧张素原(AGT)基因启动子区 5 '远侧端的单核苷酸多态性 (SNP)。在非变性胶上 ,发现不同样品的PCR产物 (双链DNA分子 )电泳迁移率不同 ;而在变性胶上 ,对应的单链DNA分子电泳迁移率相同 ;PCR产物直接测序的结果表明 ,在同一PCR扩增片段中同时存在两个SNPs位点 (G 10 74T ,A 1179G) ;通过 4 76个不同样品的实验结果 ,进一步证实两个SNPs位点处于完全的连锁不平衡关系。GenBank同源性比较结果显示 :A 1179G为中国汉族人群所特有
引用
收藏
页码:219 / 222+227 +227
页数:5
相关论文
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