魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景

被引：23

作者：

孔晓瑜

姜艳艳

相建海

喻子牛

刘亚军

机构：

[1] 青岛海洋大学教育部海水养殖重点实验室

[2] 中国科学院海洋研究所

来源：

海洋科学 | 2001年 / 12期

关键词：

魁蚶(Scapharcabroughtonii); 16SrDNA; COI; 序列测定;

D O I：

暂无

中图分类号：

Q959.223.14 [];

学科分类号：

摘要：

Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.

引用

页码：46 / 48

页数：3