芍药苷对牛Ⅱ型胶原诱导的小鼠关节炎模型T细胞作用机制研究

被引：8

作者：

蔡力

张晗

齐渊元

张立丽

黄晓婉

聂红

机构：

[1] 上海交通大学医学院,上海市免疫学研究所

来源：

中国免疫学杂志 | 2014年 / 30卷 / 11期

关键词：

芍药苷; CIA; Th1; Th17;

D O I：

暂无

中图分类号：

R-332 [医用实验动物学]; R285.5 [中药实验药理];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100806 [中药药理学];

摘要：

目的:探讨芍药苷(Paeoniflorin)对牛Ⅱ型胶原诱导的小鼠关节炎模型(Collagen-induced arthritis,CIA)T细胞作用机制。方法:采用牛Ⅱ型胶原诱导DBA/1J小鼠建立CIA模型,二次免疫时随机分为两组,一组用芍药苷治疗,另一组用磷酸盐缓冲液治疗作为对照,每天进行临床评分;发病高峰期处死小鼠,HE染色法观察关节病理学变化;3H-甲基胸腺嘧啶(3H-TdR)掺入法检测小鼠脾脏细胞和CD4+T细胞增殖反应;流式细胞技术检测T细胞亚群比例;酶联免疫吸附试验(ELISA)检测小鼠脾脏细胞培养上清中炎性因子分泌情况。结果:芍药苷治疗组小鼠临床评分显著低于对照组(P<0.05);治疗组CD3抗体和CD28抗体刺激的脾脏细胞和CD4+T细胞增殖能力明显减弱(P<0.05),治疗组CII刺激的脾脏细胞和CII特异性CD4+T细胞增殖能力明显减弱(P<0.01);治疗组Th1和Th17细胞亚群比例低于对照组(P<0.01);治疗组脾脏细胞培养上清中IFN-γ和IL-17含量低于对照组(P<0.05)。结论:芍药苷可能通过抑制致病性T细胞增殖,降低Th1和Th17细胞亚群比例,减少炎性细胞因子分泌,从而缓解CIA病情。

引用

页码：1485 / 1489

页数：5

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