猪水泡病病毒全基因组核苷酸序列的测定与分析

被引：7

作者：

冶贵生

刘湘涛

张彦明

马玉花

张淼涛

韩雪清

张永国

谢庆阁

机构：

[1] 中国农业科学院兰州兽医研究所畜禽病毒学重点开放实验室,中国农业科学院兰州兽医研究所畜禽病毒学重点开放实验室,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,中国农业科学院兰州兽医研究所畜禽病毒学重点开放实验室,西北农林科技大学动物科技学院,中国农业科学院兰州兽医研究所畜禽病毒学重点开放实验室兰州　西北农林科技大学动物科技学院杨凌　,兰州　,杨凌　,杨凌　,杨凌　,兰州　,杨凌　,兰州

来源：

病毒学报 | 2005年 / 01期

关键词：

猪水泡病病毒; 全基因组; 序列分析;

D O I：

10.13242/j.cnki.bingduxuebao.001677

中图分类号：

S852.659.6 [];

学科分类号：

090601 ;

摘要：

Six overlapping DNA fragments from swine vesicular disease virus (SVDV) RNA were acquired by RT-PCR,nested PCR,and half-nested PCR.These fragments were individually cloned and sequenced.Comparing and analyzing of homologies of nucleotide and animo acid sequences by computer software,the DNA sequence homologies were 98.4% with SVDV J1′73,98.2% with SVDV H/3′76,98.2% with SVDV(Seechurn.P,et al),91.0% with SVDV NET/1/92;the amino acid sequence homoiogies were 98.9% with SVDV J1′73,98.9% with SVDV H/3′76,99.0% with SVDV (Seechurn P,et al.)96.9% with SVDV NET/1/92.

引用

页码：69 / 71

页数：3

共 5 条

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