二袖套法建立恒河猴原位肝移植的稳定模型

被引：6

作者：

冉江华 ^{[1
,2
]}

李铸 ^{[1
,2
]}

刘静 ^{[1
,2
]}

张升宁 ^{[1
,2
]}

吴淑媛 ^{[1
,2
]}

张熙冰 ^{[1
,2
]}

李来邦 ^{[1
,2
]}

李立 ^{[1
,2
]}

张鸿青 ^{[1
,2
]}

刘滇生 ^{[1
,2
]}

机构：

[1] 昆明市第一人民医院肝胆胰一病区

[2] 昆明医学院附属甘美医院

来源：

中国组织工程研究与临床康复 | 2011年 / 15卷 / 05期

关键词：

二袖套法; 肝移植; 动物模型; 恒河猴; 血管重建;

D O I：

暂无

中图分类号：

R657.3 [肝及肝管]; R-332 [医用实验动物学];

学科分类号：

摘要：

背景:以往多应用大鼠建立肝移植模型对肝移植术后排斥反应进行基础研究,而灵长类非人类大动物建立肝移植模型能更接近临床肝移植的需求。目的:探讨建立稳定的恒河猴原位肝移植模型的最佳方案。方法:选用健康恒河猴进行同种异体原位肝移植20次,以10只雄性作为受体,10只雌、雄不限为供体,借鉴临床肝移植和各种动物模型建立的方法,使用二袖套法加肝动脉重建建立稳定的恒河猴原位肝移植模型。结果与结论:20次恒河猴原位肝移植模型成功率90%,供肝切取时间(17±3)min,供肝修整时间(35±5)min,受体移植时间(133±45)min,受体无肝期(12±4)min,术后24h存活率90%(18/20),72h存活率为80%(16/20),1周存活率50%(10/20),14只分别于术后2周内死于急性排斥反应,最长存活38d,也死于急性排斥反应,所有受体均无门静脉血栓形成及胆道并发症发生。改进后的恒河猴同种异体原位肝移植模型具有操作简便、手术成功率高、重复性和稳定性好的优点,是肝移植临床前期研究的较理想动物模型。

引用

页码：762 / 768

页数：7

共 12 条

[1] Surgical techniques of arterialized orthotopic liver transplantation in rats[J]. MA Yi WANG Guo-dong GUO Zhi-yong GUO Zhi-gang HE Xiao-shun CHEN Gui-hua Organ Transplantation Center,First Hosnital,Sun Yat-sen University,Guangzhou 510080,China.Chinese Medical Journal. 2007(21)
[2] Effect of operation-synchronizing transfusion of apoptotic spleen cells from donor rats on acute rejection of recipient rats after liver transplantation[J]. Jing Liu, Yi Gao, Shuan Wang, Er-Wei Sun, Yu Wang, Zhi Zhang, Yi-Qiang Shan, Department of Organ Transplantation, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China Shi-Zheng Zhong, Institute of Clinical Anatomy, First Military Medical University, Guangzhou 510515, Guangdong Province, China.Worl
[3] Improved two-cuff technique for orthotopic liver transplantation in rat
Yong Peng
Department of General Surgery
[J]. Hepatobiliary & Pancreatic Diseases International, 2004, (01) : 33 - 37
[4] Successful arterial thrombolysis and percutaneous transluminal angioplasty for early hepatic artery thrombosis after split liver transplantation in a four-month-old baby
Lopez-Benitez, R.
Schlieter, M.
Hallscheidt, P. J.
Radeleff, B. A.
Kauffmann, G.
Richter, G. M.
Schmidt, J.
Engelmann, G.
[J]. PEDIATRIC TRANSPLANTATION, 2008, 12 (05) : 606 - 610
[5] A Novel Technique for Rat Liver Transplantation Using Quick Linker System: A Preliminary Result[J] . Graziano Oldani,Marcello Maestri,Annalisa Gaspari,Ettore Lillo,Giacomo Angelastri,Luca Matteo Lenti,Johannes Rademacher,Mario Alessiani,Paolo Dionigi.Journal of Surgical Research . 2008 (2)
[6] Acute Rejection After Liver Transplantation: Is There a Specific Immunological Pattern?[J] . O. Perrella,C. Sbreglia,G. Arenga,A. Perrella,A. Ferrara,A. D’Antonio,G. Di Costanzo,L. Atripaldi,C. Alone,D. Sciano,O. Cuomo.Transplantation Proceedings . 2006 (10)
[7] Novel magnetic rings for rapid vascular reconstruction in canine liver transplantation model
Shi, Y.
Lv, Y.
Wang, B.
Zhang, Y.
Jiang, A.
Li, J. H.
Zhang, X. F.
Li, Q. Y.
Meng, K. W.
Liu, C.
Yu, L.
Pan, C. E.
[J]. TRANSPLANTATION PROCEEDINGS, 2006, 38 (09) : 3070 - 3074
[8] Clamping the supra-celiac aorta can effectively increase the success rate of orthotopic rat liver transplantation by increasing the tolerable time of the anhepatic phase
Liu, Chinsu
Tsai, Hsin-Lin
Chin, Taiwai
Loong, Che-Chuan
Hsia, Cheng-Yuan
Wei, Choufu
[J]. JOURNAL OF SURGICAL RESEARCH, 2006, 136 (01) : 116 - 119
[9] Piggyback technique in adult orthotopic liver transplantation: an analysis of 1067 liver transplants at a single center[J] . Seigo Nishida,Noboru Nakamura,Anil Vaidya,David M. Levi,Tomoaki Kato,Jose R. Nery,Juan R. Madariaga,Enrique Molina,Phillip Ruiz,Anthony Gyamfi,Andreas G. Tzakis.HPB . 2006 (3)
[10] Surgical techniques of orthotopic rat liver transplantation
Spiegel, HU
Palmes, D
[J]. JOURNAL OF INVESTIGATIVE SURGERY, 1998, 11 (02) : 83 - 96

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