应用RAPD技术对中国3品系实验用小型猪进行亲缘关系的分析

被引:5
作者
吴丰春
魏泓
甘世祥
王爱德
曾养志
机构
[1] 第三军医大学实验动物中心
[2] 贵阳中医学院
[3] 广西大学
[4] 云南农业大学 重庆
[5] 重庆
基金
国家自然科学基金重点项目;
关键词
Miniature pig; Phylogenetic relationship; RAPD;
D O I
暂无
中图分类号
S828.2 [];
学科分类号
090501 ;
摘要
The phylogenetic relationship was studied among three strains of Chinese laboratory miniature pig including Guizhou miniature pig,Guangxi Bama pig,Xishuangbanna inbred pig with RAPD of 100 single primers.39 polymorphic primers were selected out,in which 15 single could be used to distinguish among three strains,8 primers among Guizhou miniature pig and the other two,4 primers among Guangxi Bama miniature pig and the other two,12 primers among Xishuangbanna inbred miniature pig and the other two.By using 15 polymorphic primers,RAPD analysis showed the phylogenetic index was 0 780 between Guizhou and Guangxi Bama miniature,0 663 between Xishuangbanna inbred and Guizhou miniature Pigs.The results suggested that phylogenetic relationship was closer between Guizhou and Gangxi Bama miniature pigs than that between Xishuangbanna and the other two miniature pigs.
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收藏
页码:487 / 491
页数:5
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