5-氮杂胞苷和地塞米松对大鼠骨髓间充质干细胞分化作用的实验研究

被引：8

作者：

陈志雄

王若禺

许伟华

机构：

[1] 华中科技大学同济医学院附属协和医院骨科

来源：

中国矫形外科杂志 | 2017年 / 01期

关键词：

5-氮杂胞苷; 骨髓间充质干细胞; 糖皮质激素; 成骨分化;

D O I：

暂无

中图分类号：

R681.8 [下肢骨];

学科分类号：

100220 [骨科学];

摘要：

[目的]探究5-氮杂胞苷和地塞米松对大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)成骨分化能力的影响。[方法]SD大鼠骨髓间充质干细胞体外扩增和传代培养,流式细胞术鉴定细胞表面标志,取3代细胞随机分为空白组、激素组、激素+5-氮杂胞苷组,相应药物干预3 d后成骨诱导分化14 d,RT-PCR和Western blot检测Runx2和PPARγ的表达。[结果]所取得骨髓间充质干细胞纯度和均一性好,细胞表面标记CD90高表达,CD34、CD45低表达。第3代细胞经药物干预和成骨诱导后,与空白组相比,激素组Runx2基因表达降低,PPARγ基因升高,5-氮杂胞苷+激素组结果与激素组相反(P<0.05)。[结论]激素能抑制骨髓间充质干细胞成骨分化,促进成脂分化,5-氮杂胞苷能干预激素的这种作用,提高骨髓间充质干细胞分化潜能。

引用

页码：58 / 63

页数：6

共 12 条

[1]

5'-氮杂胞苷对激素性股骨头坏死骨髓间充质干细胞分化作用的实验研究[J] 莫峰波;杨述华;叶树楠;孙志博; 中国矫形外科杂志 2015, 02

[2]

人骨髓间充质干细胞的生物学特性及成骨诱导分化的研究[J] 代志鹏;许伟华;杨述华;刘先哲;冯勇;贾杰;冯晓波; 中国矫形外科杂志 2014, 15

[3]

Mesenchymal stem cells: Molecular characteristics and clinical applications[J] Farbod Rastegar;Deana Shenaq;Eric R Wagner;Stephanie H Kim;Russell R Reid;Hue H Luu;Rex C Haydon; World Journal of Stem Cells 2010,

[4]

The effects of PPAR-γ inhibition on gene expression and the progression of induced osteogenic differentiation of human mesenchymal stem cells[J] Cecilia Granéli;Camilla Karlsson;Helena Brisby;Anders Lindahl;Peter Thomsen Connective Tissue Research 2014,

[5]

Aberrant CpG Islands’ Hypermethylation of ABCB1 in Mesenchymal Stem Cells of Patients with Steroid-associated Osteonecrosis[J] Zhibo Sun;Shuhua Yang;Shunan Ye;Yukun Zhang;Weihua Xu;Bo Zhang;Xianzhe Liu;Fengbo Mo;Wenbin Hua The Journal of Rheumatology 2013,

[6]

Transient overexpression of Ppar&ggr;2 and C/ebpα in mesenchymal stem cells induces brown adipose tissue formation[J] Dmitriy Sheyn;Gadi Pelled;Wafa Tawackoli;Susan Su;Shiran Ben-David;Dan Gazit;Zulma Gazit Regen. Med. 2013,

[7]

Transcription Factor Runx2 and its Application to Bone Tissue Engineering[J] Luca Dalle Carbonare;Giulio Innamorati;Maria Valenti Stem Cell Reviews and Reports 2012,

[8]

Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for bone tissue engineering: Basic science to clinical translation[J] Hideaki Kagami;Hideki Agata;Arinobu Tojo International Journal of Biochemistry and Cell Biology 2010,

[9]

Regulation of bone development and extracellular matrix protein genes by RUNX2[J] Toshihisa Komori Cell and Tissue Research 2010,

[10]

Decreased in the number and function of circulation endothelial progenitor cells in patients with avascular necrosis of the femoral head[J] Yong Feng;Shu-Hua Yang;Bao-Jun Xiao;Wei-Hua Xu;Shu-Nan Ye;Tian Xia;Dong Zheng;Xian-Zhe Liu;Yun-Fei liao Bone 2009,

← 1 2 →