E.coli O157:H7 LAMP检测方法的建立

被引：6

作者：

朱海

吕敬章

范放

洪小柳

马淑棉

黄李华

刘慧玲

机构：

[1] 深圳出入境检验检疫局

来源：

分子诊断与治疗杂志 | 2010年 / 2卷 / 02期

关键词：

环介导的等温扩增; E.coliO157:H7; rfbE;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

100215 ;

摘要：

目的建立检测E.coli O157:H7环介导的等温扩增方法(LAMP)。方法选取E.coli O157:H7的rfbE基因设计LAMP引物,优化建立LAMP检测体系。并用该体系和国标法对76份人工污染样品进行了检测。结果所建立的E.coli O157:H7 LAMP体系具有良好的特异性,用该体系对9属13种41株菌进行检测,结果只有E.coli O157:H7的扩增产物电泳结果为阶梯状条带,非E.coli O157:H7均未产生扩增产物,灵敏性为2.7×101CFU/mL。样品及培养基对反应体系没有影响或影响很小。对76份样品进行了检测,E.coli O157:H7 LAMP检测方法与国标法的总符合率为96.1%,2h内即可完成检测。结论本研究建立的E.coli O157:H7的LAMP法不但解决了分子生物学检测对实验室仪器设备的高要求问题,而且检测方法简便、快捷,非常便于基层实验室和现场检测使用。

引用

页码：98 / 101

页数：4

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