猴头菇多糖对小鼠脾淋巴细胞体外增殖及细胞周期的影响

被引：24

作者：

郑乃珍

郑小香

李萍

李健

秦韬

马玉芳

黄一帆

机构：

[1] 福建农林大学中西兽医结合与动物保健福建省高校重点实验室

来源：

中国兽医科学 | 2015年 / 45卷 / 08期

关键词：

猴头菇多糖; 脾淋巴细胞; 增殖; 细胞周期;

D O I：

10.16656/j.issn.1673-4696.2015.08.012

中图分类号：

S853.7 [中兽医药物学];

学科分类号：

090604 [动物药学];

摘要：

为探索猴头菇多糖(HEP)对小鼠脾淋巴细胞体外增殖及细胞周期的影响,采用四甲基偶氮唑盐(MTT)法检测HEP单独或协同ConA、LPS对小鼠脾淋巴细胞增殖的影响;用流式细胞仪检测小鼠脾淋巴细胞周期的变化。结果显示,HEP的质量浓度在6.25～100μg/mL范围内能显著促进小鼠脾淋巴细胞增殖,且48h效果最佳;HEP与ConA或LPS共同作用小鼠脾淋巴细胞时,小鼠脾淋巴细胞的D570nm值高于ConA和LPS单独作用的,表现出明显的协同效应。流式细胞仪检测结果表明,HEP通过促进脾淋巴细胞从G0/G1期进入S期,从而促进脾淋巴细胞增殖。结果表明,HEP可单独或协同ConA、LPS促进小鼠脾淋巴细胞增殖及DNA的合成。

引用

页码：837 / 842

页数：6

共 27 条

[1]

Immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum.[J].Zhe Ren;Chenghua He;Yanhong Fan;Huimin Si;Yuwei Wang;Zhiyu Shi;Xiumei Zhao;Yating Zheng;Qingxin Liu;Haibin Zhang.International Journal of Biological Macromolecules.2014,

[2]

Optimization of ultrasonic-assisted extraction and in vitro antioxidant activities of polysaccharides from Trametes orientalis.[J].Yi Zheng;Yong Li;Wei-dong Wang.Carbohydrate Polymers.2014,

[3]

Anticancer potential of Hericium erinaceus extracts against human gastrointestinal cancers [J].

Li, Guang ;

Yu, Kai ;

Li, Fushuang ;

Xu, Kangping ;

Li, Jing ;

He, Shujin ;

Cao, Shousong ;

Tan, Guishan .

JOURNAL OF ETHNOPHARMACOLOGY, 2014, 153 (02) :521-530

[4]

Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell [J].

Huang, Yee ;

Jiang, Chunmao ;

Hu, Yuanliang ;

Zhao, Xiaojuan ;

Shi, Cheng ;

Yu, Yun ;

Liu, Cui ;

Tao, Yang ;

Pan, Huarong ;

Feng, Yibo ;

Liu, Jiaguo ;

Wu, Yi ;

Wang, Deyun .

CARBOHYDRATE POLYMERS, 2013, 96 (02) :516-521

[5]

Immunomodulatory effects of polysaccharides isolated from Hericium erinaceus on dendritic cells.[J].Shyang-Chwen Sheu;Ying Lyu;Meng-Shiou Lee;Jai-Hong Cheng.Process Biochemistry.2013,

[6]

Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey [J].

Zhang, Zuofa ;

Lv, Guoying ;

Pan, Huijuan ;

Pandey, Ashok ;

He, Weiqiang ;

Fan, Leifa .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 51 (05) :1140-1146

[7]

Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme.[J].Xiaoming Chen;Wenjian Nie;Guoqing Yu;Yali Li;Yunshuang Hu;Jianxin Lu;Liqin Jin.Food and Chemical Toxicology.2011, 3-4

[8]

Hericium erinaceum enriched diets enhance the immune response in Paralichthys olivaceus and protect from Philasterides dicentrarchi infection.[J].Ramasamy Harikrishnan;Ju-Sang Kim;Man-Chul Kim;Chellam Balasundaram;Moon-Soo Heo.Aquaculture.2011, 1

[9]

Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro [J].