双氢青蒿素逆转人肺腺癌细胞多药耐药作用研究

被引：5

作者：

陈卫强

戚好文

吴昌归

李志奎

柏长青

刘颖格

机构：

[1] 第四军医大学西京医院呼吸内科

来源：

现代肿瘤医学 | 2006年 / 03期

关键词：

双氢青蒿素; 肺癌; 顺铂; 耐药;

D O I：

暂无

中图分类号：

R734.2 [肺肿瘤];

学科分类号：

100214 ;

摘要：

目的观察双氢青蒿素和顺铂在体外对A549及A549/CDDP细胞的作用,分析双氢青蒿素抗肺癌和逆转耐药效果。方法采用细胞计数法绘制亲代A549细胞和A549/CDDP细胞生长曲线,细胞增殖及增殖抑制实验采用MTT检测法:A549/CDDP细胞分为顺铂单药作用组,联合作用组(100nM双氢青蒿素加顺铂,320nM双氢青蒿素加顺铂),序贯治疗组(100nM双氢青蒿素预处理),用MTT检测法分析计算IC50值进行比较。结果顺铂对人肺腺癌A549细胞和A549/CDDP细胞IC50值分别为0.270μg/ml和5.703μg/ml,耐药倍数为21.12。对A549/CDDP细胞,联合治疗100nM双氢青蒿素组顺铂IC50值为2.225μg/ml(逆转倍数2.56),320nM双氢青蒿素组顺铂IC50值0.464μg/ml(逆转倍数12.29)。对A549/CDDP细胞,序贯治疗组顺铂IC50为2.523μg/ml(逆转倍数2.26)。结论联合用药和序贯治疗均可逆转A549/CDDP细胞对顺铂的耐药。

引用

页码：284 / 286

页数：3

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