蛋白组学分析放射抗拒性鼻咽癌细胞差异表达蛋白

被引:5
作者
王中卫
王亚利
金迎迎
任宏涛
机构
[1] 西安交通大学医学院第二附属医院肿瘤病院放疗科
关键词
鼻咽癌; 蛋白组学; 放射抗拒性;
D O I
暂无
中图分类号
R739.63 [咽肿瘤];
学科分类号
100214 ;
摘要
目的筛选同一来源放射敏感性不同的鼻咽癌细胞蛋白组学差异表达,探讨鼻咽癌放射抗拒机制。方法建立放射敏感性不同的人鼻咽癌细胞株,采用双向凝胶电泳技术和图像分析软件PDQuest分离和分析不同阶段细胞间的差异蛋白质点,采用LC-MS/MS质谱分析系统鉴定差异蛋白质点,采用Gene Oncology Annotation将已知差异蛋白质进行分类。部分筛选蛋白质采用Western blot进行验证。结果 CNE-2和CNE-2R细胞有差异候选差异蛋白质27个,其中11个蛋白质在放射抗拒CNE-2R细胞中表达下调,16个上调。其中的4个:4-3-3σ、GADD45、PRKDC和HSP27用Western blot的方法得到了进一步确认。结论 CNE-2细胞受射线反复照射产生放射抗拒性细胞,在蛋白组学水平上发生了某些突变,并将这些突变稳定的遗传下来,从而表现出放射抗拒性的表型;通过调控其中与放射抗拒性产生相关的某些蛋白表达,就有可能调控细胞的放射敏感。4-3-3σ、GADD45、PRKDC和HSP27是预测NPC放疗敏感性的潜在基因标志物,它们表达失调可能参与NPC放射抗拒性的产生。
引用
收藏
页码:810 / 815
页数:6
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