基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究

被引：17

作者：

张洁 ^{[1
]}

严丽娟 ^{[2
]}

林琳 ^{[1
]}

陈文贵 ^{[3
]}

宋秀宇 ^{[3
]}

颜晓梅 ^{[4
]}

杭纬 ^{[1
]}

黄本立 ^{[1
]}

机构：

[1] 厦门大学化学化工学院化学系现代分析科学教育部重点实验室

[2] 厦门出入境检验检疫局技术中心

[3] 厦门市第一医院

[4] 厦门大学化学化工学院化学生物系化学生物学福建省重点实验室

来源：

高等学校化学学报 | 2008年 / 11期

基金：

中国博士后科学基金;

关键词：

糖尿病; 糖尿病肾病; 代谢组学; 超高效液相色谱; 飞行时间质谱;

D O I：

暂无

中图分类号：

R587.1 [糖尿病]; R692 [肾疾病];

学科分类号：

1002 ; 100201 ; 100210 ;

摘要：

Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid metabolism.

引用

页码：2171 / 2173

页数：3

共 2 条

[1] 液相色谱和质谱联用技术结合化学计量学应用于代谢组学的研究进展 [J].

林艳萍 ;

司端运 ;

刘昌孝 .

分析化学, 2007, (10) :1535-1540

[2]

..Salek R.M;Maguire M.L;Griffin J.L.et al;.Physiol.Genomics.2007,

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