美金刚通过调节ERK、CREB信号通路及突触可塑性保护脑缺血再灌注小鼠的作用研究

被引：17

作者：

宋加兴 ^{[1
]}

张清秀 ^{[2
]}

何磊 ^{[1
]}

魏秀娥 ^{[2
]}

机构：

[1] 徐州医科大学

[2] 徐州医科大学第二附属医院神经内科

来源：

中国比较医学杂志 | 2019年 / 06期

关键词：

脑缺血; 美金刚; 认知功能; 突触可塑性; 小鼠;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100706 [药理学];

摘要：

目的研究美金刚对脑缺血再灌注小鼠模型ERK、CREB信号通路及突触可塑性的作用机制。方法60只小鼠随机分为假手术组(Sham),模型组(MCAO),美金刚20 mg/kg组(M20),美金刚4 mg/kg组(M4),生理盐水组(NS),除假手术组外,其余4组均使用线栓法建立小鼠大脑中动脉梗死模型,观察小鼠的体重变化及神经功能缺损程度,甲酚紫染色观察小鼠脑萎缩体积,粘附物去除实验观察缺血小鼠的运动及感觉功能变化,Morris水迷宫检测小鼠的认知记忆功能,Western Blot检测ERK1/2、p-ERK1/2、CREB、p-CREB、PSD-95、Synaptophysin等蛋白的表达情况。结果与模型组(MCAO)、美金刚4 mg/kg组(M4)及生理盐水组(NS)相比,美金刚20 mg/kg组的小鼠体重下降程度减小,神经功能缺损评分下降,脑萎缩体积减轻,小鼠感觉运动功能出现改善,水迷宫实验结果提示学习记忆损伤程度减轻,p-ERK1/2、p-CREB、PSD-95、synaptophysin等蛋白表达升高。结论小鼠脑缺血后持续给予美金刚20 mg/kg治疗,可以提高小鼠的神经功能恢复,改善小鼠的学习记忆功能,改善了大脑的突触可塑性。

引用

页码：1 / 7

页数：7

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