高效液相色谱法检测泰乐菌素在肉鸡组织中的残留

被引：16

作者：

孔科

袁宗辉

范盛先

王大菊

卿柳庭

周诗其

杨尔宁

操继跃

机构：

[1] 华中农业大学畜牧兽医学院湖北武汉

[2] 华中农业大学食品科技

来源：

中国兽医学报 | 1999年 / 05期

关键词：

泰乐菌素; 肉鸡; 残留; 高效液相色谱法;

D O I：

10.16303/j.cnki.1005-4545.1999.05.022

中图分类号：

S859.84 [动物性食品中化学物残留];

学科分类号：

0906 ;

摘要：

将肉鸡的肌肉、肝脏、肾脏和脂肪组织制成匀浆，用乙腈、石油醚和二氯甲烷萃取、净化后，用乙腈定容。以乙腈－甲醇－０．００５ｍｏｌ／Ｌ磷酸二氢铵溶液（６０∶３０∶１０）为流动相，用高效液相色谱检测。本法对溶解于乙腈中的泰乐菌素的最低检测限为０．０５ｍｇ／Ｌ，在肉鸡各种组织中的最低检测限为０．１０ｍｇ／Ｌ。标准曲线的线性范围为０．１０～６．４０ｍｇ／Ｌ。泰乐菌素质量浓度为０．１０、０．８０、６．４０ｍｇ／Ｌ时的批间变异系数分别为（２．３±０．２）％、（１．９±１．３）％、（２．６±０．９）％。０．２０、０．４０、０．８０ｍｇ／Ｌ的泰乐菌素在以上４种组织的回收率均符合残留检测的要求。结果表明，本法是一种灵敏、精密、可靠的检测肉鸡组织中泰乐菌素残留的分析方法。

引用

页码：489 / 491

页数：3

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