3,3',5,5'-四甲基联苯胺-H2O2-辣根过氧化物酶伏安酶联免疫分析法测定人血清免疫球蛋白E

被引：7

作者：

何亚楠

陈洪渊

机构：

[1] 南京大学化学化工学院!南京，

来源：

高等学校化学学报 | 1997年 / 08期

关键词：

3，3'，5，5'-四甲基联苯胺（TMB）; 血清兔疫球蛋白E（IgE）; ELISA; 示差脉冲伏安法;

D O I：

暂无

中图分类号：

R446.6 [免疫学检验];

学科分类号：

100208 ;

摘要：

A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with TMB exhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgE antibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISA procedure. It has been ap-plied to the determination of IgE in human serum with satisfactory results.

引用

页码：0 / 0+0 +0

页数：3