山羊催乳素基因PCR-SSCP分析

被引:7
作者
赵秀华 [1 ]
储明星 [2 ]
王金玉 [1 ]
方丽 [2 ]
叶素成 [2 ]
机构
[1] 扬州大学动物科技学院
[2] 中国农业科学院北京畜牧兽医研究所
基金
北京市自然科学基金;
关键词
山羊; 高繁殖力; 催乳素基因; PCR-SSCP;
D O I
暂无
中图分类号
S827 [山羊];
学科分类号
090502 [动物营养与饲料科学];
摘要
设计5对引物,采用PCR-SSCP技术检测催乳素(prolactin,PRL)基因外显子1~外显子5在高繁殖力山羊品种(济宁青山羊、波尔山羊、文登奶山羊)和低繁殖力山羊品种(内蒙古绒山羊)中的单核苷酸多态性,同时研究该基因对济宁青山羊高繁殖力的影响。通过克隆测序首次获得了山羊PRL基因外显子全序列。结果表明PRL基因在研究的4个山羊品种中都不存在单核苷酸多态性,提示检测的PRL基因座位对济宁青山羊高繁殖力没有显著影响。
引用
收藏
页码:35 / 38
页数:4
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