肺癌患者外周血树突状细胞的生物学特性研究

被引：4

作者：

黄建安

朱一蓓

古涛

席泓

王天立

戴俊

张学光

机构：

[1] 苏州大学附属第一医院

[2] 苏州大学医学生物技术研究所

来源：

中国肿瘤生物治疗杂志 | 2003年 / 03期

关键词：

肺癌; 树突状细胞; 外周血单个核细胞; CD40;

D O I：

暂无

中图分类号：

R734.2 [肺肿瘤];

学科分类号：

摘要：

目的 :探讨肺癌患者外周血来源的树突状细胞 (dendriticcells,DC)生物学特性。方法 :从肺癌患者外周血分离获得单个核细胞 ,用细胞因子GM CSF (10 0 μg/L) ,IL 4 (5 0 μg/L)体外培养诱导。凋亡肿瘤细胞负载 2 4h后加入TNF α(10μg/L)或CD4 0激发型单抗于培养DC中 ,继续诱导 3～ 4d。分别测定DC的表型、DC摄取抗原能力 ;混合淋巴细胞反应(MLR)对T细胞增殖能力的测定 ;细胞计数和3 H TdR掺入观察DC对T细胞的激发和扩增效应 ,并与健康志愿者外周血来源的DC进行比较。结果 :患者外周血单个核细胞和正常人外周血单个核细胞来源的DC均高表达CD1α ,CD83,CD80 ,CD86和HLA DR等DC的相关抗原和共刺激分子 ;患者的未成熟DC能有效摄取FITC Dextran ,经TNF α或CD4 0激发型单抗激发诱导后 ,成为成熟和有功能的DC ,几乎完全失去对抗原的摄取能力 ,与健康人外周血来源DC组相比无明显差别 (P >0 .0 5 ) ;患者单个核细胞来源的DC在体外具有激发自体和同种异体外周血T细胞增殖的能力。结论 :肺癌患者的外周血来源单个核细胞可以诱导成为具有功能的成熟DC。

引用

页码：175 / 179

页数：5

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