产超广谱β内酰胺酶大肠杆菌的检测及其耐药表型和基因型

被引:21
作者
沈定霞
JonesRN
吴坚
徐雅萍
机构
[1] 解放军总医院微生物科
[2] 美国Iowa大学医学院病理系医学微生物室
关键词
大肠杆菌; 耐药性; 基因型;
D O I
暂无
中图分类号
R378 [病原细菌];
学科分类号
摘要
目的 研究临床分离的产超广谱 β内酰胺酶 (extendedspectrumβlactamases,ESBLs)大肠杆菌对常用抗生素的耐药性及其基因类型。方法 应用双纸片扩散法及Etest检测ESBLs ;微量稀释法测定产ESBLs大肠杆菌对临床常用抗生素的耐药性 ;全自动核型分析和脉冲场凝胶电泳 (pulsedfieldgelelectrophoresis,PFGE)研究产ESBLs大肠杆菌的基因型。结果  2 3株产ESBLs大肠杆菌共有 12种耐药表型 ,4种核型及 9种脉冲场凝胶电泳分型。耐药表型中 ,以对庆大霉素、妥布霉素、环丙沙星、四环素及TMP SMZ同时耐药组合最常见 ,占 39 1% ;基因分型中 ,核型 PFGE型 :2 42 5 2与 2 42 5 4各 4株分别分离自肝胆外科和血液科 ,3株 880 1 1a分离自高级病房 ,其余基因型呈散在分布于多个病房。结论 产ESBLs大肠杆菌对常用抗生素具有多种耐药表型 ;核型分析及脉冲场凝胶电泳是进行产ESBLs细菌引起医院感染流行病学研究的有效手段。
引用
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页码:93 / 96
页数:4
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