血管生成因子在子宫内膜异位症中的作用

被引：18

作者：

辛晓燕

李洁

严瑞兰

王德堂

机构：

[1] 第四军医大学西京医院妇产科!西安

来源：

现代妇产科进展 | 2001年 / 03期

关键词：

子宫内膜异位症; 上皮细胞; 血小板源生长因子; 血管内皮生长因子;

D O I：

10.13283/j.cnki.xdfckjz.2001.03.010

中图分类号：

R711.71 [子宫内膜异位症];

学科分类号：

100211 ;

摘要：

目的 :探讨血小板衍生内皮细胞生长因子 (PDGF)对体外培养的子宫内膜异位症患者子宫内膜腺上皮细胞的影响 ,及血管内皮生长因子 (VEGF)在内异症发生中的作用。方法 :对 11例子宫内膜异位症患者的子宫内膜组织进行体外培养、纯化、鉴定。将上皮细胞接种于 96孔板中 ,用加有不同浓度的PDGF处理。用MTT法观察PDGF对腺上皮细胞的作用并用计算机灰度分析仪分析VEGF的免疫组化结果。结果 :PDGF对腺上皮细胞有明显的促增殖作用 ,10ng ml时最明显 (P <0 .0 5) ,在不经过和经过PDGF刺激的腺上皮细胞中都有VEGF表达 ,但刺激后VEGF的表达明显增强 (P <0 .0 5)。结论 :PDGF和VEGF作为血管生成因子 ,可能通过自分泌和旁分泌的途径参与子宫内膜异位症的发生与发展

引用

页码：187 / 189+241 +241

页数：4

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