高胆固醇对内皮祖细胞数量和功能的影响

被引：25

作者：

朱军慧

王兴祥

陈君柱

陶谦民

朱建华

孙坚

机构：

[1] 浙江大学医学院第一附属医院心内科暨心血管病研究所,浙江大学医学院第一附属医院心内科暨心血管病研究所,浙江大学医学院第一附属医院心内科暨心血管病研究所,浙江大学医学院第一附属医院心内科暨心血管病研究所,浙江大学医学院第一附属医院心内科暨心血管病研究所,浙江大学医学院第一附属医院心内科暨心血管病研究所杭州,杭州,杭州,杭州,杭州,杭州

来源：

中华内科杂志 | 2004年 / 04期

关键词：

高胆固醇血症; 干细胞; 显微镜检查,共焦;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

目的　观察高胆固醇血症患者外周血内皮祖细胞 (EPC)数量和功能的变化。方法选择高胆固醇血症患者和非高胆固醇血症患者各 2 0例 ,用密度梯度离心法从外周血获取单个核细胞 ,将其接种在人纤维连接蛋白包被培养板 ,培养 7d后贴壁细胞进行细胞化学分析。激光共聚焦显微镜鉴定FITC UEA Ⅰ和DiI acLDL双染色阳性细胞为正在分化的EPC。采用MTT比色法、改良的Boyden小室和黏附能力测定实验观察EPC的增殖、迁移和黏附能力。结果　高胆固醇血症患者外周血EPC数量明显减少 [(41 8± 8 7比 6 4 5± 16 6 )EPCs/× 2 0 0视野 ,P <0 0 5 ],且EPC数量与血总胆固醇水平 (r =- 0 6 5 9,P <0 0 0 1)和低密度脂蛋白胆固醇水平 (r =- 0 6 11,P <0 0 0 1)呈反向线性关系。高胆固醇血症患者EPC的增殖、迁移和黏附能力也明显受损。结论　高胆固醇患者外周血内皮祖细胞数量减少、功能减退。

引用

页码：24 / 27

页数：4

共 10 条

[1]

Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease. Vasa M,Fichtlscherer S,Aicher A,et al. Circulation Research . 2001

[2]

Expression of VEGFR-2 and AC133 by circulating human CD34 ( + ) cells identifies a population of functional endothelial precursors. Peichev M,Naiyer AJ,Pereira D,et al. Blood . 2000

[3]

Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo. Murayama T,Tepper OM,Silver M,et al. Experimental Hematology . 2002

[4]

Neoendothelialization after peripheral blood stem cell transplantation in humans: a case report of a Tokaimura nuclear accident victim. Suzuki T,Nishida M,Futami S,et al. Cardiovascular Research . 2003

[5]

Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. Hill JM,Zalos G,Halcox JP,et al. The New England Journal of Medicine . 2003

[6]

Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease. Vasa M,Fichtlscherer S,Adler K,et al. Circulation . 2001

[7]

Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Kalka C,Masuda H,Takahashi T,et al. Proceedings of the National Academy of Sciences of the United States of America . 2000

[8]

Isolation of putative progenitor endothelial cells for angiogenesis. Asahara T,Murohara T,Sullivan A,et al. Science . 1997

[9]

Statin therapy accelerates reendothelialization: a novel effect involving mobilization and incorporation of bone marrow-derived endothelial progenitor cells. Walter DH,Rittig K,Bahlmann FH,et al. Circulation . 2002

[10]

Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury. Werner N,Junk S,Laufs U,et al. Circulation Research . 2003

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