基于尤斯灌流室技术的泽泻汤大鼠肠吸收研究

被引：8

作者：

朱蕴 ^{[1
,2
]}

顾星 ^{[1
,2
]}

张兵 ^{[1
,2
]}

祁东利 ^{[1
,2
]}

刘志东 ^{[1
,2
]}

魏子斌 ^{[1
,2
]}

机构：

[1] 天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室培育基地

[2] 天津中医药大学现代中药发现与制剂技术教育部工程研究中心

来源：

天津中医药大学学报 | 2017年 / 36卷 / 04期

关键词：

泽泻汤; 尤斯灌流室; LC-MS/MS; 白术内酯Ⅱ; 白术内酯Ⅲ;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

[目的]研究泽泻汤中成分白术内酯Ⅱ和Ⅲ在大鼠不同肠段的跨膜吸收特征。[方法]采用Agilent Zorbax Plus C18色谱柱,流动相为乙腈-0.1%甲酸水（42∶58）,流速:0.3 mL/min。质谱电喷雾离子源,阳离子MRM模式,白术内酯Ⅱ和Ⅲ的定量离子分别为m/z233.2→m/z187,m/z249.15→m/z231。尤斯灌流室法考察泽泻汤在大鼠离体十二指肠、空肠、回肠、结肠的吸收,接收侧样品10 000 r/min离心15 min后用液相色谱-质谱色谱法（LC-MS/MS）分析。[结果]白术内酯Ⅱ在大鼠不同肠黏膜的表观渗透系数范围在6.10～25.22×10-6 cm/s之间,白术内酯Ⅲ在1.88～5.41×10-6cm/s之间,两者最佳吸收部位是结肠。[结论]该方法专属性好,准确度高,可用于泽泻汤中白术内酯Ⅱ和Ⅲ的大鼠肠吸收研究。

引用

页码：299 / 302

页数：4

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