栀子苷通过抑制细胞凋亡保护糖尿病大鼠胰岛细胞

被引：12

作者：

谭小梅

机构：

[1] 重庆医科大学附属第一医院綦江医院/重庆市綦江区人民医院消化内分泌科

来源：

临床和实验医学杂志 | 2017年 / 16卷 / 08期

关键词：

大鼠; 栀子苷; 糖尿病; 凋亡;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的探索栀子苷(GEN)对糖尿病大鼠胰岛细胞的影响及相关机制。方法利用链脲佐菌素(STZ)制作大鼠糖尿病模型,50只SD大鼠随机分为5组,对照组,模型组,栀子苷10 mg/kg,栀子苷25 mg/kg,栀子苷50 mg/kg,每组10只,连续栀子苷灌胃用药1周后,对照组和模型组只给与等量生理盐水灌胃处理。检测大鼠空腹血糖值;利用Western blot法检测凋亡因子NF-κB以及凋亡蛋白Bax、Bcl-2表达;酶联免疫吸附(ELISA)法检测Caspase-3、Caspase-9活性。结果一周后,模型组血糖值显着高于对照组,而栀子苷组较模型组有明显降低(P<0.05);Western blot显示,模型组NF-κB和Bax表达显着高于对照组,而栀子苷组则明显抑制NF-κB和Bax表达,并呈剂量依赖效应(P<0.05),相反,模型组中Bcl-2表达则显着低于对照组,而栀子苷组则显着高于模型组(P<0.05);ELISA显示,模型组Caspase-3、Caspase-9活性显着高于对照组,而栀子苷组则明显抑制Caspase-3、Caspase-9活性,并呈剂量依赖效应(P<0.05)。结论栀子苷对糖尿病大鼠模型具有降糖作用,可能是通过抑制NF-κB和Bax,提高Bcl-2表达,同时还可能抑制Caspase-3和Caspase-9蛋白酶活性,从而减轻了胰岛细胞的凋亡来发挥作用。

引用

页码：741 / 743

页数：3

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