流式细胞分选技术在微生物表面展示文库筛选中的应用进展

被引:8
作者
辛忠涛
机构
[1] 军事医学科学院基础医学研究所北京
关键词
流式细胞分选; 微生物; 表面展示文库;
D O I
10.13309/j.cnki.pmi.2003.03.018
中图分类号
Q93-3 [微生物研究与微生物实验];
学科分类号
摘要
流式细胞分选技术具有测量速度快、统计学精度高、可在分析的同时把具有指定特征的细胞分选出来等特点 ,已广泛应用到微生物 (细菌、酵母、噬菌体和杆状病毒等 )表面展示文库的筛选。筛选配基的类型涵盖抗原模拟表位、特异性单链抗体或单链T细胞受体、酶变异体以及蛋白酶抑制剂等。本文对这方面的研究进展作一综述
引用
收藏
页码:62 / 66
页数:5
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