血浆血栓素B2放射免疫分析法

被引:83
作者
李振甲
杨梅芳
郝秀华
王仁芝
方永鑫
宋家云
机构
[1] 解放军总医院
[2] 军事医学科学院
关键词
血浆血栓素; 放射免疫分析法; B2; 石油醚; 溶剂油; 乙酸乙醋;
D O I
暂无
中图分类号
学科分类号
摘要
本文报告了血浆血栓素B2的放射免疫分析法。样品经酸化后用乙酸乙酯提取,再经石油醚提取,排除了脂肪干扰,提高了方法的准确性。抗血清以TXB2-BSA为免疫原注射兔制备。[3H]-TXB2的比放射性为140ci/mmol。放射免疫分析采用非平衡法,提高了灵敏度。本法主要技术指标如下:标准曲线范围为2.5~160pg/管,三种不同浓度血浆批内CV分别为6.4、7.8、7.3%。提取示踪回收为90.3%,冷回收为92.3%。健康成人血浆测定值为127±48pg/ml。
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页码:35 / 38
页数:4
相关论文
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