吴茱萸碱诱导人黑色素瘤A375-S2细胞的两种死亡机制

被引：18

作者：

张莹

吴立军

田代真一

小野寺敏

池岛乔

不详

机构：

[1] 沈阳药科大学中日医药研究所

[2] 沈阳药科大学天然药物研究室

[3] 昭和药科大学病态科学教研室

[4] 沈阳药科大学中日医药研究所辽宁沈阳

[5] 辽宁沈阳

[6] 东京-

[7] 日本国

[8] 辽宁沈阳

来源：

药学学报 | 2003年 / 09期

关键词：

吴茱萸碱; 人黑色素瘤A375-S2; 凋亡; 抗肿瘤活性;

D O I：

10.16438/j.0513-4870.2003.09.003

中图分类号：

R73-36 [治疗实验];

学科分类号：

摘要：

目的　研究吴茱萸碱诱导A375 S2细胞死亡机制。方法　MTT法测定吴茱萸碱对A375 S2的细胞毒作用。通过倒置光显微镜 ,荧光染色 ,DNA电泳观察细胞形态学变化。应用流式细胞分析技术研究药物对细胞周期的影响。结果　吴茱萸碱明显抑制A375 S2生长 ,在 2 4h前可诱导A375 S2凋亡 ,亚二倍体峰出现 ,caspase蛋白酶被激活 ,2 4h后启动坏死途径 ,caspase蛋白酶抑制剂不能抑制细胞死亡。结论　吴茱萸碱诱导A375 S2死亡时早期启动了caspase依赖性的非经典凋亡途径 ,后期则启动了坏死途径

引用

页码：650 / 653

页数：4

共 12 条

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