烫伤对小鼠葡萄糖调节蛋白78表达的影响

被引：3

作者：

陈德昌

川上正人

李红江

冈田芳明

机构：

[1] 第二军医大学长征医院解放军急救医学中心!上海

[2] 日本国防卫医科大学附属医院救急部

来源：

中国危重病急救医学 | 1999年 / 12期

关键词：

烧伤; 小鼠; 葡萄糖调节蛋白; 皮肤; 肝脏; 器官损害;

D O I：

暂无

中图分类号：

R644 [烧伤及烫伤（灼伤）];

学科分类号：

摘要：

目的：研究烫伤对小鼠各器官葡萄糖调节蛋白７８（ＧＲＰ７８）表达的影响。方法：Ｃ５７ＢＬ／６小鼠，体重２０～２５ｇ，制成２０％体表面积Ⅲ度背部烫伤模型。烫伤后０．５、１、２４和７２小时采集肝、脾、肾、烫伤和未烫伤皮肤，以及肺、脑和右大腿肌肉，抽提各器官总ＲＮＡ，以逆转录多聚酶链式反应检测烫伤后各器官内ＧＲＰ７８表达情况。结果：①肝脏：烫伤后１小时ＧＲＰ７８表达明显增加（Ｐ＜０．０５），２４小时仍高于对照组，伤后７２小时恢复至对照组水平；②皮肤：烫伤后０．５小时表达无明显增加，１小时烫伤组表达明显超过对照组（Ｐ＜０．０５），伤后２４小时表达仍高于对照组，于７２小时恢复至对照组水平。③其他脏器：烫伤后ＧＲＰ７８在脾、肾、脑、肺和肌肉内表达与对照组无显著差异。结论：烫伤能诱导皮肤、肝脏内ＧＲＰ７８基因大量表达。

引用

页码：747 / 749

页数：3

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