短柄五加(Acanthopanax brachypus)rbcL基因的结构分析

被引:2
作者
严华军
吴乃虎
机构
[1] 北京大学
[2] 中国科学院发育生物学研究所 博士生
[3] 北京
关键词
短柄五加(Acanthopanax brachypus); rbcL基因; DNA序列; 启动区; 反向重复序列;
D O I
10.16058/j.issn.1005-0930.1995.04.007
中图分类号
Q943 [植物细胞遗传学];
学科分类号
071007 ; 090102 ;
摘要
克隆了含完整短柄五加rbcL基因的3.2kb EcoRI片段,测定了该基因的核苷酸序列.所测核苷酸序列总长度为1924bp,其中编码区1428bp,编码475个氨基酸的蛋白质.测定的基因5’上游区共278bp,包含原核性质-35区(TTGCGC),-10区(TACAAT)及类似真核的TATA box元件(TATATA).5’前导区长194bp,其中SD序列为GGAGG,紧邻起始密码子上游.测定的3’下游区共218bp,含2个相邻的转录后可形成茎环结构的反向重复序列.短柄五加rbcL基因编码区推导的氨基酸序列与烟草、菠菜、豌豆、苜蓿、玉米、水稻、松树、地钱、衣藻和Anacystis的同源性分别为93.5%、94.11%、94.53%、94.74%、89.68%、92.21%、92.21%、92.63%、87.58%和80.84%.本文还对不同植物rbcL基因的启动区及部分5’和3’非编码区进行了比较分析.
引用
收藏
页码:54 / 62
页数:9
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