痛泻要方干预腹泻型肠易激综合征肝郁脾虚证模型大鼠的效应评价

被引：80

作者：

张北华 ^{[1
]}

王微 ^{[2
]}

王凤云 ^{[1
]}

唐旭东 ^{[1
]}

机构：

[1] 中国中医科学院西苑医院

[2] 北京市中西医结合医院

来源：

中华中医药杂志 | 2018年 / 33卷 / 10期

关键词：

腹泻型肠易激综合征; 肝郁脾虚证; 痛泻要方; 5-羟色胺; 脑源性神经营养因子; 肥大细胞; 嗜铬细胞;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理]; R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100806 [中药药理学];

摘要：

目的:探讨痛泻要方对腹泻型肠易激综合征(IBS-D)肝郁脾虚证大鼠模型的干预作用及其机制。方法:SD大鼠随机分为正常组、模型组、中药组,每组10只。采用母子分离联合慢性束缚应激和番泻叶灌胃法建立IBS-D肝郁脾虚证病证结合大鼠模型,采用自制测压计检测各组大鼠的痛觉阈值,观察造模结束后的大便积分,采用旷场实验、糖水偏好实验及悬尾实验观察大鼠行为学特征,采用流式细胞仪分析血液中T淋巴细胞亚群,间苯三酚法检测血清D-木糖含量,ELISA法检测血清免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、5-羟色胺(5-HT)、脑源性神经营养因子(BDNF)水平,甲苯胺蓝改良染色法检测近端结肠组织中的肥大细胞(MC),酶免疫组化法检测近端结肠和末端回肠组织中的嗜铬细胞(EC)。结果:与正常组相比,模型组大鼠痛觉阈值明显降低,大便积分增加,旷场穿格数、站立数明显减少,糖水偏好率降低,悬尾不动时间延长(P<0.05);血IgA水平升高,T淋巴细胞CD3+CD4+亚群比例升高,CD3+CD8+亚群比例降低,CD4+/CD8+比值升高(P<0.05);血清D-木糖含量降低、5-HT水平升高(P<0.05);近端结肠和末端回肠EC数量增加,近端结肠MC数量增加(P<0.05)。与模型组比较,中药组大鼠的痛觉阈值升高,旷场穿格数、站立数升高,糖水偏好率升高,悬尾不动时间缩短(P<0.05);血清5-HT水平降低,末端回肠EC数量减少,近端结肠MC数量减少(P<0.05)。结论:痛泻要方可降低IBS-D肝郁脾虚证模型大鼠的内脏敏感性,改善其抑郁焦虑样行为,降低血清5-HT水平,减少肠组织中EC和MC的数量。

引用

页码：4341 / 4346

页数：6

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